TBX 21基因多态性与汉族儿童哮喘易感性的相关性研究  

Relationship between TBX 21 gene polymorphism and susceptibility to asthma in Han children

作　　者：李会娟 甄兴刚 张曼[2] LI Hui-juan;ZHEN Xing-gang;ZHANG man(Henan Children's Hospital,Zhengzhou Children's Hospital,Department of Respiration,Children's Hospital Affiliated to Zhengzhou University,Zhengzhou,Henan 450000,China;不详)

机构地区：[1]河南省儿童医院(郑州儿童医院),郑州大学附属儿童医院呼吸科,河南郑州450000 [2]郑州大学第一附属医院小儿内科,河南郑州450000

出　　处：《中华全科医学》2023年第1期84-87,共4页Chinese Journal of General Practice

基　　金：河南省医学科技攻关联合共建项目(LHGJ20190928)。

摘　　要：目的探讨汉族儿童T-box转录因子21(TBX 21)基因多态性,并分析其与哮喘易感性的相关性。方法选取2019年1月—2021年1月河南省儿童医院收治的汉族哮喘患者190例,设为哮喘组,另选取同期本院健康体检的汉族儿童135名作为对照组。采集口腔黏膜标本,分析rs9910408、rs16947078、rs2240017基因位点单核苷酸多态性(SNP),比较2组3个基因位点基因型分布及等位基因频率,采用logistic回归分析研究TBX 21基因rs2240017位点多态性与哮喘易感性的关系。结果Hardy-Weinberg平衡检验结果显示,rs9910408、rs16947078、rs2240017基因型在2组儿童中的分布频率未发生偏移,研究对象代表性良好。哮喘组与对照组rs2240017基因型等级分布,差异有统计学意义(P<0.05),且哮喘组C等位基因频率显著高于对照组(89.47%vs.40.37%,P<0.05),G等位基因频率显著低于对照组(10.53%vs.59.63%,P<0.05)。2组rs9910408、rs16947078基因型等级分布,差异无统计学意义(均P>0.05)。经logistic回归分析,TBX 21基因rs2240017的G/G、C/G、C/C基因型及G等位基因频率降低、C等位基因频率升高可能是哮喘易感性的影响因素(P<0.05)。结论TBX 21基因rs2240017位点多态性与哮喘易感性密切相关。Objective To explore the polymorphism of T-box transcription factor 21(TBX 21)in children of Han nationality and analyze its association with asthma susceptibility.Methods A total of 190 Han asthma patients admitted to the paediatrics department of Henan Children's Hospital from January 2019 to January 2021 were selected as the asthma group,and 135 Han children who underwent physical examination in our hospital during the same period were selected as the control group.Oral mucosa specimens were collected;single nucleotide polymorphisms(SNP)were analysed at rs9910408,rs16947078 and rs2240017 gene loci,and the genotype distribution and allele frequencies of the 3 loci,including the TBX 21 gene,were compared between the two groups.The relationship between rs2240017 locus polymorphism and asthma susceptibility was analysed by logistic regression.Results The Hardy-Weinberg balance test showed that the distribution frequencies of rs9910408,rs16947078 and rs2240017 genotypes in the two groups of children did not shift,and the research subjects were well represented.The difference in rs2240017 genotype distribution between the asthma group and control group was statistically significant(P<0.05),and the frequency of the C allele in the asthma group was significantly higher than that of the control group(89.47%vs.40.37%,P<0.05).Moreover,the frequency of the G allele was significantly lower than that of the control group(10.53%vs.59.63%,P<0.05).No statistically significant difference in the rs9910408 and rs16947078 genotypes distribution was observed between the two groups(P>0.05).Logistic regression analysis showed that the decrease of G/G,C/G,C/C genotypes and G allele frequencies,as well as the increase of C allele frequency of TBX 21 gene rs2240017 may be the influencing factor of asthma susceptibility(P<0.05).Conclusion The rs2240017 polymorphism of the TBX 21 gene is closely related to asthma susceptibility.

关 键 词：哮喘 T-box转录因子21 儿童 基因位点单核苷酸多态性 易感性 

分 类 号：R562.25[医药卫生—呼吸系统] R446.7[医药卫生—内科学]