横经席不同部位提取物药效学评价研究  

Study on Pharmacodynamic Evaluation of Extracts from Different Parts of Calophylli Herba

作　　者：黄源春 郑志远 吕林艳 蒙永明 梁云飞 刘洋 

机构地区：[1]广西梧州制药(集团)股份有限公司,广西梧州543000 [2]广西天然药物现代制剂技术工程研究中心,广西梧州543000

出　　处：《大众科技》2022年第12期87-90,38,共5页Popular Science & Technology

基　　金：梧州市科技计划项目(2022A04021)。

摘　　要：目的:评价横经席不同部位提取物的抗细菌性炎症及改善脂肪肝的作用。方法:以受精后3天的斑马鱼用于横经席样品抗细菌性炎症功效最大检测浓度测定及其功效评价;以受精后5天的斑马鱼用于改善脂肪肝功效评价。结果:横经席根茎和横经席枝叶抗细菌性炎症功效最大检测浓度均为125μg·m L^(-1),横经席种子抗细菌性炎症功效最大检测浓度为2000μg·m L^(-1);横经席根茎和横经席枝叶31.2μg·m L^(-1)、62.5μg·m L^(-1)和125μg·m L^(-1)及横经席种子500μg·m L^(-1)、1000μg·m L^(-1)和2000μg·m L^(-1)浓度组炎症部位中性粒细胞数量较模型对照组减少,具有统计学差异。横经席根茎125μg·m L^(-1)浓度组、横经席枝叶62.5μg·m L^(-1)和125μg·m L^(-1)浓度组及横经席种子1000μg·m L^(-1)和2000μg·m L^(-1)浓度组肝脏染色强度较模型对照组显著降低,具有统计学差异。结论:横经席根茎、横经席枝叶和横经席种子均具有抗细菌性炎症、改善脂肪肝功效。Objective:To evaluate the antibacterial inflammation and the improvement of fatty liver with the extracts from different parts of Calophylli herba.Methods:Zebrafishes of 3 days after fertilization were used to determine the maximum test concentration of antibacterial inflammation and the evaluation of efficacy;zebrafishes of 5 days after fertilization were used to evaluate the efficacy of improving fatty liver.Results:The maximum detectable concentration of antibacterial inflammatory efficacy of rhizomes,branches and leaves of Calophylli herba is 125μg·m L^(-1),while the maximum detectable concentration of antibacterial inflammatory efficacy of Calophylli herba seeds is 2000μg·m L^(-1).Compared with the model control group,the number of neutrophils in inflammatory sites is decreased in the Calophylli herba rhizomes,branches and leaves of 31.2μg·m L^(-1),62.5μg·m L^(-1)and 125μg·m L^(-1),and in the Calophylli herba seeds of 500μg·m L^(-1),1000μg·m L^(-1)and 2000μg·m L^(-1)groups,which is statistically different.The liver staining intensities in the rhizomes of Calophylli herba with 125μg·m L^(-1)concentration group,the branches and leaves of Calophylli herba with 62.5μg·m L^(-1)and125μg·m L^(-1)concentration group,and the seeds of Calophylli herba with 1000μg·m L^(-1)and 2000μg·m L^(-1)concentration group are significantly lower than those of the model control group,which is statistically different.Conclusion:The rhizomes,branches and leaves of Calophylli herba and the seeds of Calophylli herba have the effect of antibacterial inflammation and the improving fatty liver.

关 键 词：横经席 提取物 斑马鱼 细菌性炎症 脂肪肝 

分 类 号：R282[医药卫生—中药学]