荧光光谱法分别测定铒和镱的多吡啶配合物与牛血清白蛋白的相互作用  

The interaction of polypyridine complexes of Er and Yb with bovine serum albumin determined by fluorescence spectroscopy

作　　者：赵晓飞 张培芳 谢承志[3] 徐靖源[3] Zhao Xiaofei;Zhang Peifang;Xie Chengzhi;Xu Jingyuan(Department of Clinical Pharmacy,The Second Affiliated Hospital of Xing Tai Medical College,Xingtai 054000,China;Department of Nursing,The Second Affiliated Hospital of Xing Tai Medical College,Xingtai 054000,China;College of Pharmacy,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]邢台医学高等专科学校第二附属医院临床药学科,邢台054000 [2]邢台医学高等专科学校第二附属医院护理部,邢台054000 [3]天津医科大学药学院,天津300070

出　　处：《分析仪器》2023年第1期105-111,共7页Analytical Instrumentation

摘　　要：目的:分别研究了稀土元素铒(Er)和镱(Yb)的多吡啶配合物与牛血清白蛋白(Bovine serum albumin,BSA)的相互作用,揭示金属药物在体内的分布、转运和代谢过程,为研究作用机制提供实验依据。方法:利用荧光光谱法探讨两种稀土多吡啶配合物与BSA的猝灭机制、结合常数、结合位点数及作用方式。结果:两种配合物能够有效的导致BSA内源荧光静态猝灭;荧光光谱结果显示两种配合物的结合常数均达到10^(4)L·mol^(-1)数量级,且随着温度的升高逐渐降低;两种配合物与BSA的结合位点数约为1,表明形成1:1的非共健复合物;通过热力学参数推断出两种配合物与BSA之间主要靠疏水作用力(ΔH>0,ΔS>0);同步荧光结果表明,两种配合物的加入使BSA的构象发生变化,影响了色氨酸残基的微环境。结论:铒和镱的多吡啶金属配合物均与BSA有很好的结合能力,且结合后改变了BSA的构象,为今后成为药物提供了可能性,也为进一步研究此类稀土配合物代谢动力学打下了基础。The quenching mechanism,binding constants,number of binding sites and action mode of two rare earth polypyridine complexes with BSA were investigated by fluorescence spectroscopy.The two complexes could effectively induce the static quenching of BSA endogenous fluorescence.The fluorescence spectra showed that the binding constants of the two complexes reached the order of 104L·mol-1,and gradually decreased with the increase of temperature.The number of binding sites between the two complexes and BSA was about 1,indicating the formation of 1:1 non-co-healthy complex.According to the thermodynamic parameters,the interaction between the two complexes and BSA mainly depended on the hydrophobic force(ΔH>0,ΔS>0).The simultaneous fluorescence results showed that the conformation of BSA was changed by the addition of the two complexes,which affected the microenvironment of tryptophan residues.The polypyridine complexes of Er and Yb has good binding ability to BSA,and the conformation of BSA is changed after binding,which provides a possibility for future use as drugs.

关 键 词：稀土金属铒和镱 多吡啶 配合物 牛血清白蛋白 荧光光谱 

分 类 号：O657.3[理学—分析化学] TQ460.1[理学—化学]