一测多评法同时测定沙芪浓缩丸中5种黄酮  

Simultaneous determination of five flavonoids in Shaqi Concentrated Pills by QAMS

作　　者：萨出拉 王秀枝 杨立国 包书茵[1] 奥乌力吉[1] SA Chu-la;WANG Xiu-zhi;YANG Li-guo;BAO Shu-yin;Ao-wu-li-ji(Inner Mongolia Minzu University,Tongliao 028000,China;Inner Mongolia Autonomous Regional Research Institute of Mongolian Medicine,Hohhot 010020,China;Tongliao Vocational College,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学,内蒙古通辽028000 [2]内蒙古自治区蒙医药研究所,内蒙古呼和浩特010020 [3]通辽职业学院,内蒙古通辽028000

出　　处：《中成药》2023年第2期381-386,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82004070,82160814);内蒙古自治区科技计划项目(2020GG0103);内蒙古自治区高等学校科学研究项目(NJZY21641);内蒙古自治区高等学校“青年科技英才支持计划”(NJYT23134);蒙药研发国家地方联合工程研究中心开放基金项目(MDK2020001,MDK2021023)。

摘　　要：目的 建立一测多评法同时测定沙芪浓缩丸中毛蕊异黄酮苷、水仙苷、银锻苷、异鼠李素、芒柄花素的含量。方法 该药物90%甲醇(含1%甲酸)提取液的分析采用Welch LP-C18色谱柱(250 mm×4.6 mm, 5μm);流动相乙腈-0.4%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长260 nm。以毛蕊异黄酮苷为内标,计算其他4种黄酮的相对校正因子,计算其含量。结果 5种黄酮在各自范围内线性关系良好(r≥0.999 9),平均加样回收率91.90%~99.82%,RSD 0.26%~0.64%。一测多评法所得结果与外标法接近(RAD<2%)。结论 该方法准确可行,可用于沙芪浓缩丸的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS) method for the simultaneous content determination of calycosin-7-glucoside, narcissoside, tiliroside, isorhamnetin and formononetin in Saqi Concentrated Pills.METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Welch LP-C18column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.4% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 260 nm. Calycosin-7-glucoside was used as an internal standard to calculate the relative correction factors of the other four flavonoids, after which the content determination was made. RESULTS Five flavonoids showed good linear relationships within their own ranges(r≥0.999 9), whose average recoveries were 91.90%-99.82% with the RSDs of 0.26%-0.64%. The result obtained by QAMS approximated those obtained by external standard method(RAD<2%).CONCLUSION This accurate and feasible method can be used for the quality control of Shaqi Concentrated Pills.

关 键 词：沙芪浓缩丸 毛蕊异黄酮苷 水仙苷 银锻苷 异鼠李素 芒柄花素 一测多评 

分 类 号：R927.2[医药卫生—药学]