急性心肌梗死患者血清中LncRNA TUG1的表达及意义  

Expression and clinical significance of serum long noncoding RNA taurine upregulated gene 1 in patients with acute myocardial infarction

作　　者：赵会连[1] 牛津津 张静[2] 李涛 Zhao Huilian;Niu Jinjin;Zhang Jing;Li Tao(Third Department of Cardiovascular Medicine,First People's Hospital of Pingdingshan City,Pingdingshan 1467000,China;不详)

机构地区：[1]平顶山市第一人民医院心血管内三科,平顶山467000 [2]平顶山市第一人民医院心内重症监护室,平顶山467000 [3]阜外华中心血管病医院,郑州451464

出　　处：《中国循证心血管医学杂志》2022年第11期1349-1351,1356,共4页Chinese Journal of Evidence-Based Cardiovascular Medicine

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20190809)。

摘　　要：目的探讨长链非编码RNA(LncRNA)牛磺酸上调基因1(TUG1)在急性心肌梗死(AMI)患者血清中的表达及临床意义。方法选取2018年3月至2020年10月于平顶山市第一人民医院收治的AMI患者(AMI组)145例作为研究对象,另选取同期在我院体检的健康志愿者(健康对照组)150例作为对照。采用实时荧光定量PCR(qRT-PCR)法检测血清LncRNA TUG1水平,酶联免疫(ELISA)法检测血清心肌损伤指标肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白I(cTnI)水平;Pearson法分析AMI组受试者血清LncRNA TUG1水平与CK-MB、cTnI水平的相关性;受试者工作特征(ROC)曲线评估血清LncRNA TUG1水平对AMI的诊断价值。结果与健康对照组相比,AMI组受试者血清LncRNA TUG1、CKMB、cTnI水平均显著升高(P<0.05);AMI组受试者血清LncRNA TUG1水平与CK-MB、cTnI水平均呈显著正相关(r=0.563、r=0.485,P<0.05);血清LncRNA TUG1水平诊断AMI的ROC曲线下面积为0.975(95%CI:0.956~0.995),最佳截断值为1.415,敏感度为96.6%,特异性为90.7%,约登指数为0.873。结论LncRNA TUG1在AMI患者血清中表达上调,与心肌损伤指标水平关系密切,对AMI早期诊断具有一定参考价值。Objective To discuss the expression and clinical significance of serum long noncoding RNA taurine upregulated gene 1(LncRNA TUG1)in patients with acute myocardial infarction(AMI).Methods AMI patients(n=145,AMI group)and healthy controls(n=150,control group)were chosen from the First People’s Hospital of Pingdingshan City from Mar.2018 to Oct.2020.The level of serum LncRNA TUG1 was detected by using qRT-PCR,and levels of serum indexes of myocardial injury including creatine kinase isoenzyme(CK-MB)and cardiac troponin I(cTnI)were detected by using ELISA.The correlation among LncRNA TUG1,CK-MB and cTnI was analyzed by using Pearson method in AMI group.The diagnostic value of LncRNA TUG1 level to AMI was analyzed by using receiver operating characteristic(ROC)curve.Results The levels of serum LncRNA TUG1,CKMB and cTnI all increased significantly in AMI group compared with control group(P<0.05).The level of serum LncRNA TUG1 was significantly correlated to CK-MB(r=0.563)and cTnI(r=0.485)in AMI group(P<0.05).The area under the ROC curve of LncRNA TUG1 in diagnosing AMI was 0.975,(95%CI:0.956~0.995),optimal cutoff value was 1.415,sensitivity was 96.6%,specificity was 90.7% and Youden index was 0.873.Conclusion The expression of LncRNA TUG1 is up-regulated in AMI patients,which is closely related to indexes of myocardial injury and has some reference value to early diagnosis of AMI.

关 键 词：急性心肌梗死 血清 长链非编码RNA牛磺酸上调基因1 

分 类 号：R542.22[医药卫生—心血管疾病]