外膜蛋白ompC过表达提高大肠杆菌对庆大霉素敏感性研究  

Study on the Enhancement of the Sensitivity of Escherichia coli to Gentamicin by the Overexpression of Outer Membrane Protein ompC

作　　者：马月龙 赖泽迎 冯荆城 林少青 陈雅娟[1] MA Yue-long;LAI Ze-ying;FENG Jing-cheng;LIN Shao-qing;CHEN Ya-juan(Key Laboratory of Cellular Stress Response and Metabolic Regulation in Fujian Universities/College ofLife Science,Fujian Normal University,Fuzhou,Fujian 350108,China)

机构地区：[1]细胞逆境响应与代谢调控福建省高校重点实验室/福建师范大学生命科学学院,福建福州350108

出　　处：《福建农业科技》2022年第12期21-25,共5页Fujian Agricultural Science and Technology

基　　金：福建省自然科学基金面上项目(2019J01278)。

摘　　要：为了寻找新的药物结合敏感靶点解决致病菌耐药性难题,通过构建大肠杆菌外膜蛋白ompC过表达菌株,利用庆大霉素对其进行杀伤试验并与野生型大肠杆菌进行效果比较,通过免疫印迹法探究ompC蛋白表达量与庆大霉素杀伤效果的关系,并进一步测定过表达菌株MIC,结果表明:过表达ompC能显著增强大肠杆菌对庆大霉素的敏感性,过表达ompC后菌株W3110-pCA24N(ompC)的最小抑菌浓度8 ug·mL^(-1)明显低于W3110-pCA24N(野生型)的16 ug·mL^(-1),庆大霉素更多地被摄入大肠杆菌胞内,增强了庆大霉素的杀菌效果。因此该外膜蛋白有望作为新的候选药敏靶点。In order to find new drugs combined with the sensitive targets to solve the problem of drug resistance of pathogenic bacteria,by constructing the overexpression strain of the outer membrane protein ompC of Escherichia coli,the killing effect of gentamicin on it was tested and compared with that of the wild-type Escherichia coli.Then,the relationship between the protein expression level of ompC and the killing effect of gentamicin was explored by using the immunoblot assay,and the over-expression strain MIC was further determined.The results showed that the overexpression of ompC could significantly enhance the sensitivity of Escherichia coli to gentamicin,and after the overexpression of ompC,the minimum inhibitory concentration of the strain W3110-pCA24N(ompC)was 8 ug·mL^(-1),which was significantly lower than that of the strain W3110-pCA24N(wild type)(being 16 ug·mL^(-1)).The gentamicin was more intaken into the cells of Escherichia coli,which enhanced the bactericidal effect of gentamicin.Therefore,the outer membrane protein was expected to be a new candidate target of drug sensitivity.

关 键 词：大肠杆菌 外膜蛋白 抗生素 庆大敏素 敏感性 

分 类 号：R446.5[医药卫生—诊断学]