细胞外ATP对壳聚糖诱导的叶片活性氧及相关生理反应的影响  

Effects of extracellular ATP on chitosan-induced ROS and related physiological responses in leaves

作　　者：杨德丽 王馨 李丽[1] 达晓伟 贾凌云[1] 冯汉青[1] YANG De-li;WANG Xin;LI Li;DA Xiao-wei;JIA Ling-yun;FENG Han-qing(College of Life Sciences,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《兰州大学学报（自然科学版）》2022年第6期789-795,共7页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(31870246,31560070);甘肃省科技重大专项项目(22ZD1NA001);甘肃省引导科技创新发展专项(2019ZX-05);甘肃省重点研发计划项目(18YF1NA051);甘肃省教育科技创新项目优秀研究生“创新之星”项目(2021CXZX-214)。

摘　　要：以野生型和植物胞外ATP受体缺失突变体(dorn1-3)拟南芥植株为材料,探讨胞外ATP对壳聚糖引起的植株叶片活性氧(ROS)水平、气孔开度以及光系统Ⅱ变化的影响.结果表明,100μg/mL壳聚糖处理下两种类型拟南芥叶片内ROS均显著升高,野生型植株ROS水平升高幅度高于dorn1-3突变型.25~100μg/mL壳聚糖处理会引起野生型拟南芥叶片气孔关闭,且存在浓度梯度效应,但在相应浓度的壳聚糖处理下,不会引起dorn1-3突变型拟南芥叶片中气孔关闭;200μg/mL壳聚糖处理下突变型拟南芥叶片气孔开度降低.与对照组相比,在较低浓度壳聚糖处理下叶片的实际光合效率(Y(Ⅱ))、经过光系统Ⅱ的相对电子传递效率(ETR)和光化学淬灭系数(q P)值都升高,且突变型植株升高的幅度大于野生型;在高浓度壳聚糖处理下,拟南芥叶片Y(Ⅱ)、ETR和q P趋于下降.表明细胞外ATP可能参与调控壳聚糖诱导的ROS、气孔开度以及叶绿素荧光参数的变化.The effects of extracellular ATP on reactive oxygen species(ROS)levels,stomatal opening and photosystemⅡin leaves of Arabidopsis thaliana were investigated using wild-type and extracellular ATP receptor-deficient mutant(dorn1-3).It was found that ROS in leaves of wild-type and dorn1-3 mutant were significantly increased under 100μg/mL chitosan treatment,and ROS level of wild-type plants was higher than that of dorn1-3 mutant.In the range of 25 to 100μg/mL,chitosan treatment induced stomatal closure in wild-type leaves with a concentration gradient effect,but did not induce stomatal closure in dorn1-3 mutant leaves under the corresponding chitosan treatment.The stomata of mutant A.thaliana leaves decreased after treatment with 200μg/mL chitosan.Compared with the control,effective photosystemⅡquantum yield(Y(Ⅱ)),electron transport rate(ETR)and photochemical quenching coefficient(q P)values of leaves were increased under the treatment of chitosan at a lower concentration,and the increase rate of mutant plants was greater than that of wild-type plants.Y(Ⅱ),ETR and q P of A.thaliana leaves tended to decrease under a high concentration of chitosan.These results suggested that extracellular ATP might be involved in regulating the changes of chitosan-induced ROS,stomatal opening and chlorophyll fluorescence parameters.

关 键 词：壳聚糖 胞外ATP 活性氧 气孔 叶绿素荧光参数 

分 类 号：Q945.79[生物学—植物学]