UPLC法测定库鲁木提草成分及其指纹图谱研究  

Determination of Composition and Fingerprint Analysis of Rhizoma Kulumuti with UPLC

作　　者：木尼热·阿布都克力木 阿卜杜拉·玉苏普 魏翠梅[2] 木合塔尔·吐尔洪 Munira Abudukeremu;Abdullah Yusuf;Wei Cuimei;Muhetaer Tuerhong(College of Chemistry and Environmental Sciences,Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry,KashgarUniversity,Kashgar 844006,China;LANLIKE Chemical Electronic High Technology Co.Ltd.,Tianjin 300000,China)

机构地区：[1]喀什大学化学与环境科学学院,新疆喀什844006 [2]天津市兰力科化学电子高技术有限公司,天津300000

出　　处：《南开大学学报（自然科学版）》2022年第6期98-104,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：新疆维吾尔自治区高校科研计划重点项目(XJEDU20171010);国家自然科学基金(21864016)。

摘　　要：运用超高效液相色谱(ultrahigh performance liquid chromatography,缩写为UPLC)技术建立快速、高效、全面评价中药库鲁木提草质量的指纹图谱方法.流动相用乙腈-0.3%磷酸水溶液对样品进行梯度洗脱,流速设置为0.32 mL/min,检测波长为220 nm,柱温为30℃,进样量为2μL,采用Waters公司Acquity UPLC BEH C18(1.7μm, 2.1 mm×100 mm)色谱柱,共计分析了10个不同地区中库鲁木提草的UPLC色谱图,初步建立了库鲁木提草UPLC指纹图谱,运用中药色谱指纹图谱相似度评价系统进行分析,共确定了12个共有峰,通过与标准物质保留时间的对比进行分析,初步确认了其中的3个化合物.结果表明本方法为中药库鲁木提草提供了操作简便、快速、重复性好的质量评价方法,可为中药库鲁木提草的质量控制提供一定的参考.Ultrahigh performance liquid chromatography(UPLC) was used as a rapid, efficient and comprehensive fingerprint method for evaluating the quality of Rhizoma kulumuti. The mobile phase was selected with acetonitrile-0.3% phosphoric acid aqueous solution for gradient elution at the flow rate of0.32 m L/min with the detection wavelength of 220 nm, column temperature of 30 ℃, injection volume of2 μL. A Waters Acquity UPLC BEH C18 column(1.7 μm, 2.1 mm×100 mm) was used to obtain the UPLC chromatograms of S. Kurumuti samples obtained from 10 different regions, while the UPLC fingerprint of S. Kurumuti was initially established. A total of 12 common peaks were identified and used to record the Chinese medicine chromatographic fingerprint with similarity evaluation system. 3 compounds were preliminarily identified by comparing the retention time with the standard reference materials. The results showed that UPLC method provided a simple, rapid, and reproducible quality evaluation method for Chinese medicine Kurumuti and the strategy could provide a certain reference for the quality control of Chinese medicine Kurumuti.

关 键 词：超高效液相色谱技术 库鲁木提草 指纹图谱 质量评价 

分 类 号：O657[理学—分析化学]