丙酮酸钠对一次性力竭运动焦虑大鼠的神经保护作用  

Neuroprotective effects of sodium pyruvate on anxiety resulting from acute exhaustive exercise in rats

作　　者：张君 杨延平 冯琳 邱俊强[1,2] Zhang Jun;Yang Yanping;Feng Lin;Qiu Junqiang(Exercise Science School,Beijing Sport University;Beijing Sports Nutrition Engineering Research Center,Beijing 100084,China)

机构地区：[1]北京体育大学运动人体科学学院 [2]运动营养北京市高等学校工程研究中心,北京100084

出　　处：《神经解剖学杂志》2022年第6期634-640,共7页Chinese Journal of Neuroanatomy

基　　金：国家重点研发专项(2018YFC2000600);中央高校基本科研业务费专项基金资助课题(2021QN028);北京体育大学校自主课题(2022YB001);康宝莱冬季运动营养研究中心资助课题(KBL2019003,KBL2021007)。

摘　　要：目的:本研究使大鼠预先补充丙酮酸钠后进行运动应激来评估丙酮酸钠的抗焦虑作用,并探讨可能的机制。方法:将32只雄性SD大鼠随机分为对照组(control)、丙酮酸钠组(PYR)、运动应激组(SS)、运动应激+丙酮酸钠组(SS+PYR)。丙酮酸钠给药7 d后测量抓力并且进行大鼠一次性力竭性游泳测试,记录力竭时间。通过旷场实验、新奇抑制摄食和新物体识别测试评估大鼠焦虑症状和认知能力;通过血气分析测试大鼠血糖和血乳酸水平;检测大鼠海马活性氧簇(ROS)、丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)以及甘油三酯(TG)的水平或活性;利用Western Blot测定大鼠海马NF-κB(p65)、胶质纤维酸性蛋白(GFAP)和离子钙结合衔接蛋白1(Iba-1)的水平。结果:与control组大鼠相比,SS组大鼠进入中心区域的次数和时间显著下降(P<0.05),同时它们进入中心区域潜伏时间(P<0.05)和摄食潜伏时间(P<0.001)显著增加,另外SS组大鼠的新物体识别指数显著下降(P<0.05)。与SS组相比,补充丙酮酸钠显著逆转了这些行为缺陷(P<0.05),但对于识别新物体能力无显著作用(P>0.05)。相比于control组大鼠,SS组大鼠的血糖水平显著下降(P<0.01),乳酸和TG水平显著升高(P<0.05)。SS组大鼠的海马ROS(P<0.05)和MDA水平(P<0.01)显著升高,同时SS组大鼠的GSH和SOD活性显著下降(P<0.05)。与control组相比,SS组大鼠海马NF-κB、GFAP和Iba-1水平显著升高(P<0.05)。补充丙酮酸钠显著逆转了这些指标。结论:丙酮酸钠可能通过阻止能量代谢紊乱,改善氧化应激和神经炎症从而缓解力竭运动后大鼠的焦虑样行为。Objective: This study aimed to investigate whether sodium pyruvate has anxiolytic effects in rats exposed to sports stress, and to explore the underlying mechanisms. Methods: Thirty-two male SD rats were randomly divided into four groups: control group, sodium pyruvate group(PYR), sports stress group(SS), and sports stress+sodium pyruvate group(SS+PYR). An acute exhaustive swimming test was performed after 7-day sodium pyruvate treatments and then exhaustion time and muscle strength were measured. Open field test, novelty suppressed feeding(NSF) test, and novel object recognition test were performed to measure anxiety-like behaviors and cognitive abilities;Glucose and lactate levels were measured by blood gas analysis. The levels or activities of ROS, MDA, GSH, and SOD in the hippocampus were measured, as well as serum TG levels. The levels of NF-κB, GFAP, and Iba-1 in the hippocampus were measured by Western Blot. Results: The SS group significantly decreased the time and entries spent in the center area as well as the novel object recognition index(P<0.05), and increased latency time to enter the center area(P<0.05) and latency time in the NSF(P<0.001) compared with the control group. While these behavioral deficiencies were significantly reversed by sodium pyruvate treatments except for the novel object recognition index(P<0.05). Glucose levels were significantly decreased(P<0.01), and lactate and TG levels were significantly increased in the SS group compared with the control group(P<0.05). ROS and MDA levels were significantly increased(P<0.05 and P<0.01) and GSH and SOD activities were significantly decreased in the SS group relative to the control group(P<0.05). The levels of NF-κB, GFAP, and Iba-1 were significantly increased in the hippocampus in the SS group in comparison with the control group(P<0.05). While sodium pyruvate significantly reversed these indications. Conclusion: The present study demonstrated that sodium pyruvate may alleviate anxiety-like behaviors after the acute exhaustive exercise

关 键 词：一次性游泳力竭运动 丙酮酸钠 焦虑 氧化应激 神经炎症 大鼠 

分 类 号：G804[文化科学—运动人体科学]