运动疲劳对大鼠焦虑情绪及脑内星形胶质细胞的影响  

The effects of exercise-induced chronic fatigue on anxiety and brain astrocyte in rats

作　　者：张晓晓 张博威 刘敬祺 叶瑞琪 祁金顺[3] 孙丽娜[1,2] Zhang Xiaoxiao;Zhang Bowei;Liu Jingqi;Ye Ruiqi;Qi Jinshun;Sun Lina(College of P.E.and Sports,Beijing Normal University,Beijing 100875;State Key Laboratory of Cognitive Neuroscience and Learning,Beijing Normal University,Beijing 100875;Department of Physiology,Key Laboratory of Cellular Physiology,Ministry of Education,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]北京师范大学体育与运动学院 [2]北京师范大学认知神经科学与学习国家重点实验室,北京100875 [3]山西医科大学生理学系,细胞生理学教育部重点实验室,太原030001

出　　处：《神经解剖学杂志》2022年第6期664-670,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(32000835);北京师范大学认知神经科学与学习国家重点实验室开放课题(CNLZD2104)。

摘　　要：目的:探讨运动疲劳导致大鼠焦虑样行为的神经免疫调控机制。方法:采用随机数字法将雄性Wistar大鼠分为对照组(control)和运动疲劳组(Fatigue),选用三级递增跑台训练方案,建立慢性力竭运动疲劳模型。利用旷场实验(OFT)评估大鼠的焦虑样行为,采用免疫组织化学染色观察大鼠下丘脑和海马区胶质纤维酸性蛋白(GFAP)的表达,采用RT-PCR检测海马区GFAP和白介素-6(IL-6)mRNA的表达。结果:与对照组大鼠相比,疲劳组大鼠在旷场箱中央区域活动的距离显著减少(P<0.01),在中央区域停留的时间明显缩短(P<0.05)。免疫组织化学染色结果显示,疲劳组大鼠下丘脑、海马区内GFAP染色变深,阳性染色胞体肥大、突起增多,呈活化反应性状态。统计结果表明,疲劳组大鼠下丘脑内单位面积阳性细胞的数量显著多于对照组大鼠(P<0.05),海马区GFAP染色光密度值显著高于对照组大鼠(P<0.01)。RT-PCR结果显示,疲劳组大鼠海马区GFAP mRNA表达水平较对照组大鼠显著升高(P<0.01),IL-6 mRNA表达量也大幅升高(P<0.05)。结论:运动疲劳可导致大鼠产生焦虑样行为,其作用机制可能与下丘脑、海马区星形胶质细胞活化并分泌IL-6有关。Objective: To study the neuroimmunological regulatory mechanism of anxiety-like behavior induced by exercise-induced chronic fatigue in rats. Methods: Male Wistar rats were divided into control group(control) and fatigue group(Fatigue) by random number method. The exercise-induced chronic fatigue model was established by using three-level treadmill training. Using open field test(OFT) to assess the anxiety-like behavior of rats. Immunohistochemistry was used to determine the expression of glial fibrillary acidic protein(GFAP) in the hypothalamus and hippocampus of rats. RT-PCR was used to test the expression of GFAP mRNA and interleukin-6(IL-6) mRNA in the hippocampus of rats. Results: In OFT, compared with control, the moving distance in the central area of fatigue was significantly reduced(P<0.01), and the time staying in the central area was also significantly shortened(P<0.05). Immunohistochemical staining showed that the GFAP in the hypothalamus and hippocampus of fatigue became hyperchromatic, and the positive staining cells showed hypertrophy and increased protrusions, showing activated state. In hypothalamus, GFAP positive cells in fatigue was significantly higher than that in control(P<0.05). In hippocampus, the optical density in fatigue was significantly higher than that in control(P<0.01). RT-PCR showed that the expression of GFAP mRNA in the hippocampus of fatigue was significantly higher than control(P<0.01), and the expression of IL-6 mRNA was also significantly increased(P<0.05). Conclusion: Exercise-induced chronic fatigue can cause anxiety-like behavior in rats, and its mechanism may be related to the activation and secretion of IL-6 by astrocytes in hypothalamus and hippocampus.

关 键 词：运动疲劳 焦虑情绪 星形胶质细胞 白介素-6 海马 下丘脑 大鼠 

分 类 号：G804.8[文化科学—运动人体科学]