单倍体育种技术研究进展及其在苜蓿等豆科牧草中的应用  

Advances in Haploid Breeding Technology and Its Application in Alfalfa and Other Legume Forages

作　　者：王娜 姜腾 王彬锡 牛丽芳[1] 林浩[1] Na Wang;Teng Jiang;Binxi Wang;Lifang Niu;Hao Lin(Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Hebei Normal University,Shijiazhuang 050024,China)

机构地区：[1]中国农业科学院生物技术研究所,北京100081 [2]河北师范大学,石家庄050024

出　　处：《植物学报》2022年第6期756-763,共8页Chinese Bulletin of Botany

基　　金：国家重点研发计划(No.2022YFF1003204);海南省崖州湾种子实验室(No.B21HJ0215);呼和浩特市“政产学研推用银”创新联合体项目(No.RC2022-联合体-1)。

摘　　要：双单倍体育种通过单倍体诱导与加倍技术相结合实现遗传材料的快速纯合,显著加快纯系的选育进程和效率,是农业生物育种中的共性关键技术。该文简要回顾了诱导植物单倍体产生和染色体加倍的相关方法及研究进展,重点介绍了新近发展的基因编辑介导的体内单倍体诱导技术,并对单倍体育种技术在紫花苜蓿(Medicagosativa)等豆科牧草中的应用进行了探讨和展望。Doubled haploid breeding can achieve rapid homogenization of genetic material through the combination of haploid induction and doubling technology, which significantly accelerates the speed of pure line production and the selection process in hybrid breeding, is a common key technology in agricultural breeding. In this paper, we briefly review the methods and progress of plant haploid induction and chromosome doubling, introduce the latest gene editing-mediated in vivo haploid induction technology, and discuss and prospect the application of haploid breeding technology in Medicago sativa and other legumes forage.

关 键 词：单倍体育种技术 基因编辑技术 豆科牧草 苜蓿 

分 类 号：S54[农业科学—作物学]