HPLC-DPPH法在线分析远志抗氧化活性成分研究  

Rapid Screening of Antioxidant Active Ingredients in Polygalae Radix by HPLC-DPPH

作　　者：邸学[1] 曲浩源 王海波[1] 裴帅龙 鲁茂盛 Di Xue;Qu Haoyuan;Wang Haibo;Pei Shuailong;Lu Maosheng(Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《云南化工》2023年第1期47-49,共3页Yunnan Chemical Technology

基　　金：辽宁省中医药管理局中药资源普查项目(2019006);沈阳市科技局重点实验室项目(skf20174703);大学生创新创业训练计划项目X202010162032。

摘　　要：建立了远志抗氧活性成分的在线快速分析方法,为其药效物质筛选研究提供了支持。采用HPLC-DPPH在线分析方法,结合对照品保留值对照与活性测定,确定了活性成分,采用抗氧化阳性样品对照分析了活性成分的抗氧化活性强度。结果表明,远志中,3,6’—二芥子酰基蔗糖、西伯利亚远志糖酯A6为主要抗氧化活性成分。在线快速分析方法简捷、高效,适用于中药远志的抗氧化活性成分的快速筛选。To establise an on-line antioxidant screening method for rapid detection of antioxidative components in Polygala Radix for supporting pharmacodynamic studies.The antioxidative actives were detected by on-line HPLC-DPPH method,and the bioactive ingredients were qualitatively identified by compared with reference substance.The main antioxidative active ingredients are 3,6′-disinapoyl sucrose and sibricose A6.The DPPH-HPLC method is intuitive and accurate,which provides technical basis and platform support for rapid screening of antioxidant components in complex natural products.

关 键 词：远志 抗氧化成分筛选 HPLC-DPPH 

分 类 号：R284.1[医药卫生—中药学]