饲料中添加不同水平的鸡肠粉对鲤肠道菌群结构的影响  

Effects of Different Levels Chicken Intestine Meal Supplementation on the Community Structure of Intestinal Microflora of Cyprinus carpio

作　　者：彭祖想 严林 卫力博 高欣 王悦晗 翟浩杰 任同军 王伟 韩雨哲 PENG Zu-xiang;YAN Lin;WEI Li-bo(College of Fisheries and Life Science,Dalian Ocean University,Dalian,Liaoning 116023)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]大连海洋大学辽宁省北方鱼类应用生物学与增养殖重点实验室,辽宁大连116023

出　　处：《安徽农业科学》2023年第1期85-90,共6页Journal of Anhui Agricultural Sciences

基　　金：辽宁省教育厅青年科技人才“育苗”项目(QL202004);大连海洋大学“蔚蓝英才”人才计划项目。

摘　　要：为研究饲料中添加新型蛋白源鸡肠粉对鲤(Cyprinus carpio)肠道菌群的影响,选取初始体重为(57.30±0.10)g的鲤150尾,随机分为5组,每组10尾鱼,分别投喂鸡肠粉添加量为0%(D1组)、5%(D2组)、10%(D3组)、15%(D4组)、100%(D5组)的试验饲料,进行56 d养殖试验。饲养结束后,采集肠道样品,基于Illumina Miseq PE300平台进行高通量测序。结果表明,饲料中添加10%和15%的鸡肠粉降低了鲤肠道菌群的多样性,但具有改善肠道菌群结构的效果。D3、D4组α多样性显著低于D1组(P<0.05)。D3组降低了变形菌门(Proteobacteria)和邻单孢菌属(Plesiomonas)相对丰度,D3、D4组鲸杆菌属(Cetobacterium)和气单孢属(Aeromonas)相对丰度升高。表明在鸡肠粉添加量为10%时,鲤肠道致病菌相对丰度最低,有益菌相对丰度最高,对鲤肠道菌群结构有最好的改善作用。A 56 d feeding trial was conducted to evaluate the effects of chicken intestine meal on intestinal microbiota of Cyprinus carpio.A total of 150 Cyprinus carpio with an initial body weight of(57.30±0.10)g were randomly divided into 5 groups with 3 replicates per group and allocated 10 fish in each replicate.The fish were fed expeimental diets supplemented with 0%(group D1),5%(group D2),10%(group D3),15%(group D4)and 100%(group D5)chicken intestine meal.After rearing,intestinal samples were collected and high-throughput sequencing was performed based on the Illumina Miseq PE300 platform.The results showed that the addition of 10%and 15%chicken intestinal meal to the feed reduced the diversity of the carp intestinal flora,but had the effect of improving the structure of the intestinal flora.The alpha diversity of the D3 and D4 groups was significantly lower than that of the D1 group(P<0.05).The relative abundance of Proteobacteria and Pleomorphomonas was reduced in the D3 group and increased in the D3 and D4 groups for Cetobacterium and Aeromonas.The study showed that at 10%chicken intestinal meal addition,the carp intestinal pathogenic bacteria abundance was relatively lowest and the relative abundance of beneficial bacteria was highest,which had the best effect on improving the carp intestinal flora structure.

关 键 词：鸡肠粉 鲤 高通量测序 肠道菌群 

分 类 号：S963[农业科学—水产养殖]