微量热法研究MB/TiO_(2)复合物对白色念珠菌的抑制行为  

Microcalorimetric Study of MB/TiO_(2) Complex on the Inhibition Effect of Candida Albicans

作　　者：王智俊 刘东亮 付娌丽 饶璐 李月生[2,3] WANG Zhijun;LIU Dongliang;FU Lili;RAO Lu;LI Yuesheng(School of Pharmacy,Hubei University of Science and Technology,Xianning,Hubei 437100;College of Nuclear Technology and Chemical Biology,Hubei University of Science and Technology,Xianning 437100;Hubei Industrial Technology Research Institute of Intelligent Health,Hubei University of Science and Technology,Hubei University of Science and Technology,Xianning 437100)

机构地区：[1]湖北科技学院药学院,湖北咸宁437100 [2]湖北科技学院辐射化学与功能材料湖北省重点实验室,湖北咸宁437100 [3]湖北省智慧康养产业技术研究院,湖北咸宁437100

出　　处：《绵阳师范学院学报》2023年第2期34-41,共8页Journal of Mianyang Teachers＇ College

基　　金：湖北省高等学校优秀中青年科技创新团队项目(T2020022);咸宁市科技研究与开发(高新类研发重点专项)项目(2021GXYF021);湖北省大学生创新创业训练计划项目(S201910927021);湖北科技学院科学发展基金(2020TD01,2021ZX01,2022FH09)。

摘　　要：在近似生理条件下,采用微量热法研究亚甲基蓝(MB)/TiO_(2)复合物对白色念珠菌的抑制行为.测定了白色念珠菌生长代谢热功率输出曲线,根据热动力学函数得到白色念珠菌生长速率常数(k)、传代时间(t_(G))、最大产热功率(P_(m))、抑制率(I)等热动力学参数.定量说明MB/TiO_(2)复合物对白色念珠菌的抑制效果强于TiO_(2),且MB/TiO_(2)复合物的生长速率常数(k)和最大产热功率(P_(m))随着其浓度的增加而下降,而传代时间(t_(G))和抑制率(I)随着其浓度的增加而增加,此外MB/TiO_(2)复合物的生长速率常数(k)和抑制率(I)均与其浓度成一定的线性关系.The inhibitory behavior of methylene blue(MB)/TiO_(2)complex on Candida albicans was studied by a microthermal method under similar physiological conditions.The growth metabolic thermal power output curve of Candida albicans was measured.According to the thermal kinetic function,the growth rate constant(k),generation time(t_(G)),maximum heat power of growth phase(P_(m))and inhibitory ratio(I)of Candida albicans were obtained.Quantitative results showed that the inhibitory effect of MB/TiO_(2)complex on Candida albicans was more substantial than that of TiO_(2).The growth rate constant(k)and maximum heat power of growth phase(P_(m))of the MB/TiO_(2)complex decreased with the increase of its concentration.In contrast,the generation time(t_(G))and inhibitory ratio(I)increased with its concentration.In addition,the growth rate constant(k)and inhibitory ratio(I)of MB/TiO_(2)complexes are linearly related to its concentration.

关 键 词：微量热法 MB/TiO_(2) 白色念珠菌 抗菌活性 热动力学 

分 类 号：O642[理学—物理化学]