TOR抑制剂雷帕霉素对荔枝霜疫霉生长发育及自噬的影响  

Effect of TOR Inhibitor Rapamycin on Growth, Development, and Autophagy ofPeronophythora litchii

作　　者：吕林 杨成东 张雪 于戈 陈泰旭 陈庆河 LV Lin;YANG Chengdong;ZHANG Xue;YU Ge;CHEN Taixu;CHEN Qinghe(Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests,Ministry of Education/College of Plant Protection,Hainan University,Haikou,Hainan 570228,China;Sanya Nanfan Research Institute,Hainan University/Hainan Yazhou Bay Seed Laboratory,Sanya,Hainan 572025,China)

机构地区：[1]海南大学植物保护学院/热带农林生物灾害绿色防控教育部重点实验室,海南海口570228 [2]海南大学三亚南繁研究院/崖州湾种子实验室,海南三亚572025

出　　处：《福建农业学报》2022年第11期1448-1453,共6页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32160614);海南省自然科学基金创新团队项目(321CXTD437);福建省作物有害生物监测与治理重点实验室开放基金(MIMCP-202102);海南大学科研启动基金项目[KYQD(ZR)-20080]。

摘　　要：【目的】明确TOR(Target of Rapamycin)信号通路抑制剂雷帕霉素对荔枝霜疫霉生长发育、致病性及自噬的影响。【方法】采用不同浓度的TOR抑制剂雷帕霉素处理荔枝霜疫霉菌,观察不同浓度雷帕霉素对荔枝霜疫霉的菌丝生长及形态、孢子囊产生数量、游动孢子释放和致病性的影响,并通过丹酰戊二胺(MDC)染色观察细胞自噬。【结果】雷帕霉素处理后,荔枝霜疫霉的菌丝生长明显受到抑制,半最大效应浓度(EC 50)值为29.18 ng·mL^(−1),随着雷帕霉素浓度的增加,菌丝生长抑制不同程度增加;当雷帕霉素深度为25.0 ng·mL^(−1)时,菌落生长抑菌率为45.3%,孢子囊产生数量为8.0×10^(4)个·mL^(−1),仅为对照组的27.9%;雷帕霉素处理能促进游动孢子的释放;同时严重影响荔枝霜疫霉菌的致病性。通过自噬体观察表明,雷帕霉素处理后荔枝霜疫霉菌丝中自噬体的数量显著增加,促进荔枝霜疫霉的细胞自噬。【结论】TOR抑制剂雷帕霉素通过调控荔枝霜疫霉的细胞自噬,从而影响病菌的生长发育及致病性,研究结果为荔枝霜疫霉的致病机制提供科学依据。【Objective】Effects of the target of rapamycin(TOR)inhibitor rapamycin on the growth,development,pathogenicity,and autophagy of Peronophythora litchi were studied.【Method】The mycelial growth,sporangial production,zoospore release,and pathogenicity of the P.litchi after being treated by the TOR inhibitor rapanmycin in varying concentrations were determined,while the mycelial autophagy observed under a microscope with MDC staining.【Result】Rapamycin exhibited significant inhibitory effect on the mycelial growth of P.litchii with an EC 50 value of 29.18 ng·mL^(−1) and varying extents of increases with higher dosages.When treated with 25.0 ng·mL^(−1) rapamycin,the inhibition rate was 45.3%and the sporulation at 8.0×10^(4) sporangia·mL^(−1)(which was 27.9% of control),while the zoospore release increased,but the virulence of P.litchii significantly reduced.The MDC-stained autophagosomes significantly increased as well.【Conclusion】The TOR signaling pathway inhibitor rapamycin played an important role in the mycelial growth,development,and virulence of P.litchii.

关 键 词：荔枝霜疫霉 雷帕霉素 TOR抑制剂 生长发育 细胞自噬 

分 类 号：S436[农业科学—农业昆虫与害虫防治]