Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells  

作　　者：Jeonghun Baek Chun Wai Mai Wei Meng Lim Lai Chun Wong 

机构地区：[1]School of Medicine,International Medical University,Kuala Lumpur 57000,Malaysia [2]Department of Pharmaceutical Chemistry,School of Pharmacy,International Medical University,Kuala Lumpur 57000,Malaysia [3]Department of Pharmaceutical Technology,School of Pharmacy,International Medical University,Kuala Lumpur 57000,Malaysia

出　　处：《International Journal of Ophthalmology(English edition)》2023年第1期40-46,共7页国际眼科杂志（英文版）

摘　　要：AIM:To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial(ARPE)-19 cells in vitro.METHODS:ARPE-19 cells were cultured in Dulbecco’s Modified Eagle Medium-F-12 supplemented with 10%foetal bovine serum and 1%penicillin–streptomycin in a humidified 5%CO_(2) incubator maintained at 37℃.Cells were treated with 247μmol/L lutein,49μmol/L zeaxanthin and 1%(v/v)of either coconut oil,corn oil,peanut oil,olive oil,sunflower oil,soybean oil,castor oil,or linseed oil for 48h.Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography.RESULTS:Among the oils tested,the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil.CONCLUSION:ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.

关 键 词：LUTEIN ZEAXANTHIN adult retinal pigment epithelial-19 cells vegetable oil 

分 类 号：R285[医药卫生—中药学]