菌根真菌在改善附生兰干旱耐受性中的作用:以禾叶贝母兰为例  

The role of mycorrhizal fungi in improving drought tolerance of epiphytic orchid:a case study on Coelogyne viscosa

作　　者：刘娜 赵泽宇 石利欣 杨露娜 邢晓科[1] LIU Na;ZHAO Zeyu;SHI Lixin;YANG Luna;XING Xiaoke(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《菌物学报》2023年第1期217-230,共14页Mycosystema

基　　金：国家自然科学基金(32170013);中国医学科学院医学与健康科技创新工程-重大协同创新项目(2021-I2M-1-031)。

摘　　要：干旱胁迫是在多种生态系统中影响植物生存、发育及产量的最主要的非生物因素之一。菌根共生已被证明可以提高植物对干旱的耐受性。兰科植物对菌根真菌有非常高的依赖性,但是有关兰科菌根真菌是否可以提高宿主植物的耐旱性以及能提高到什么程度还少有报道。在本研究中,我们检测了一株分离自附生型兰科植物禾叶贝母兰Coelogyne viscosa的胶膜菌属真菌Tullasnella sp.hy-111对宿主植物幼苗生长及耐旱性的影响,并从转录组水平检测了该菌根真菌对禾叶贝母兰幼苗基因表达的影响。结果显示,接种hy-111不仅能显著提高幼苗的生物量、与耐旱相关的酶活性以及渗透调节物质的富集,而且还能显著诱导植物抗性途径相关基因的上调表达。本研究表明菌根真菌能改善生长于胁迫的附生生境中的兰科植物对于干旱的耐受性,并可能在兰科植物的生态适应中起到重要作用。Drought stress is one of the most important abiotic factors that affects plant survival,development and yield in many ecosystems.Mycorrhizal symbiosis has been proven to enhance drought resistance of host plants.Orchids are well known for their strong reliance on mycorrhizal fungi,but it remains unclear whether and to what extent mycorrhizal symbiosis affects drought tolerance in orchids.In this research,the effects of Tullasnella sp.hy-111 isolated from the roots of epiphytic orchid Coelogyne viscosa on the growth and drought tolerance of orchid seedlings were tested,and differences in gene expression at the transcriptome level were examined.The results showed that inoculation of hy-111 significantly enhanced biomass of seedlings and increased the activities of drought resistance related enzymes and the accumulation of osmoregulatory substances.hy-111 inoculum also significantly induced up-regulating expression of genes involved in the plant resistance pathways.These results suggest that orchid mycorrhizal fungi are capable of improving drought tolerance of orchids growing in stressful epiphytic habitats and probably play an important role in the ecological adaptation of epiphytic orchids.

关 键 词：兰科菌根 干旱胁迫 生态功能 转录组 

分 类 号：Q948.122.3[生物学—植物学]