低氧运动对肥胖大鼠BAIBA表达及白色脂肪棕色化的影响  

Effects of Hypoxic Training on Expression of BAIBA and Browning of White Fat in Obese Rats

作　　者：冯俊鹏 路瑛丽[1] 王雪冰[1,3] 张海波 冯连世 FENG Junpeng;LU Yingli;WANG Xuebing;ZHANG Haibo;FENG Lianshi(China Institute of Sport Science,Beijing 100061,China;Beijing Sport University,Beijing 100084,China;Guangxi University,Nanning 530004,China.)

机构地区：[1]国家体育总局体育科学研究所,北京100061 [2]北京体育大学,北京100084 [3]广西大学,广西南宁530004

出　　处：《中国体育科技》2023年第2期10-17,共8页China Sport Science and Technology

基　　金：国家体育总局体育科学研究所基本科研业务费资助项目(基本20-11);国家自然科学基金项目(31471139)。

摘　　要：目的:探讨低氧运动对肥胖大鼠BAIBA表达及白色脂肪棕色化的影响。方法:3周龄雄性SD大鼠100只普通饲料喂养1周后,随机分为标准饮食组(10只)和高脂饮食组(90只),经过12周肥胖造模成功后,在肥胖大鼠中选取随机选取32只,分为常氧安静组(NC组)、常氧运动组(NE组)、低氧安静组(HC组)和低氧运动组(HE组),共4组,每组各8只。NE组和HE组进行跑台运动干预,跑速分别为25 m/min和20 m/min,训练时长为1 h/天,训练频度为5天/周,共进行4周。HC组和HE组进行低氧干预,采用模拟海拔3 500 m(氧浓度13.6%)的低氧干预方案,共进行4周。最后一次运动结束后48 h进行取材,取大鼠血液、腓肠肌和腹股沟脂肪组织用于测试。使用酶法和直接法测定血脂浓度;采用HPLC-MS/MS法检测血液和腓肠肌中BAIBA浓度;采用实时荧光定量PCR和Western Blot法分别测定腹股沟脂肪组织中PPARα和UCP-1的mRNA和蛋白表达量。结果:1)NE组和HE组大鼠体质量和Lee’s指数显著低于NC组(P<0.01),HE组大鼠体质量显著低于NE组(P<0.01);2)NE组和HE组大鼠TC、TG和LDL显著低于NC组,且HE组大鼠HDL显著高于NC组(P<0.01),HE组的LDL显著低于NE组(P<0.01);3)NE组和HE组大鼠腓肠肌中BAIBA浓度显著高于NC组(P<0.01),血液中BAIBA略有升高但无显著性差异;4)NE组和HE组大鼠腹股沟脂肪组织中PPARα和UCP-1 mRNA和蛋白表达显著高于NC组(P<0.01),HE组大鼠腹股沟脂肪组织中PPARα和UCP-1蛋白表达显著高于NE组(P<0.01);5)大鼠腓肠肌BAIBA浓度与大鼠体质量、Lee’s指数、TC呈负相关关系,与大鼠腹股沟脂肪组织的PPARα和UCP-1 mRNA表达量呈正相关关系;血液BAIBA与大鼠TC呈负相关关系,与大鼠腹股沟脂肪组织的PPARα和UCP-1 mRNA表达量呈正相关关系,都具有显著性差异(P<0.05, P<0.01)。结论:4周低氧运动降低肥胖大鼠体质量、Lee’s指数,改善脂代谢;低氧运动上调肥胖大鼠骨骼肌BAIBA分泌水平;低氧运动上调�Objective: To investigate the effect of hypoxic training on the expression of BAIBA and white fat browning in obese rats. Methods: One hundred male SD rats of 3 weeks old were fed with normal diet for 1 week and then they were randomly divided into standard diet group(10 rats) and high-fat diet group(90 rats). After 12 weeks of successful obesity modelling, Thrity-five rats were randomly selected and divided into four groups: Normoxic control group(NC group), normoxic exercise group(NE group), hypoxic control group(HC group) and hypoxic exercise group(HE group). NE group and HE group were given treadmill training intervention,running speeds of 25 m/s or 20 m/s, training duration was 1 h/day, and training frequency of 5 day/week for a total of 4 weeks. Hypoxic intervention was performed in the HC and HE groups, using a hypoxic intervention program that simulated an altitude of 3 500 m(oxygen concentration 13.6%) also for 4 weeks. Samples were taken 48 h after the last training. Rat blood,gastrocnemius muscle and inguinal fat were taken for testing. The blood lipid concentration was determined by enzyme method and direct method;the concentration of BAIBA in blood and gastrocnemius muscle was detected by HPLC-MS/MS;the mRNA and protein expression of PPARα and UCP-1 in inguinal fat were detected by RT-qPCR and Western blot. Results: 1) The weight and Lee’s index of the rats in the NE group and the HE group were significantly lower than the NC group(P<0.01), and the weight of the rats in the HE group was significantly lower than the NE group(P<0.01);2) the TC, TG and LDL of NE group and HE group were significantly lower than the NC group, and the HDL of the HE group was significantly higher than that of the NC group(P<0.01), and the LDL of the HE group was significantly lower than that of the NE group(P<0.01);3) the concentration of BAIBA in the gastrocnemius of NE group and HE group were significantly higher than that in the NC group(P<0.01), and BAIBA in the blood was slightly increased but there was no significa

关 键 词：低氧运动 肥胖 BAIBA 脂代谢 棕色化 

分 类 号：G804.2[文化科学—运动人体科学]