高强度间歇训练对增龄大鼠脂肪组织衰老相关分泌表型的影响及其机制探讨  

Effects and Mechanisms of High-Intensity Intervals Training on Senescence-Associated Secretory Phenotype in Adipose Tissue of Aging Rats and Mechanisms

作　　者：马松 李方晖 吴大帅[1] 谢天 王静[1] 乔一波[1] MA Song;LI Fanghui;WU Dashuai;XIE Tian;WANG Jing;QIAO Yibo(Nanjing Normal University,Nanjing 210023,China;Zhaoqing University,Zhaoqing 526061,China)

机构地区：[1]南京师范大学,江苏南京210023 [2]肇庆学院,广东肇庆526061

出　　处：《中国体育科技》2023年第2期25-34,共10页China Sport Science and Technology

基　　金：国家自然科学基金项目(31971099);广东省省级科技计划项目(2015A020219015)。

摘　　要：目的:探讨高强度间歇训练(HIIT)对增龄大鼠脂肪组织衰老相关分泌表型(SASP)的影响及机制。方法:将18月龄雌性大鼠分为安静对照组(SED)、持续性耐力组(MICT)和HIIT组,每组12只。HIIT组起始速度为15 m/min,时间为4 min;之后进行高强度训练,速度为25 m/min,运动1 min,依次交替9个循环。MICT组以17 m/min速度运动45 min,持续8个月。免疫组化观测内脏脂肪β-半乳糖苷酶,蛋白免疫印迹和RT-qPCR检测脂肪组织p16-INK4a、p53、脂联素(Adiponectin)、瘦素(Leptin)、白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和单核细胞趋化蛋白-1(MCP-1)表达,蛋白免疫印迹检测核转录因子-κB(NF-κB)、p38丝裂原活化蛋白激酶(p38MAPK)、蛋白激酶B(AKT)、核转录因子-κB抑制蛋白α(IkBα)和c-Jun N端激酶(JNK)总蛋白及其磷酸化水平,蛋白免疫荧光观察p16-INK4a、p-NF-κB和p-lkBα蛋白含量。结果:与SED和MICT组相比,HIIT组β-半乳糖苷酶染色阳性脂肪细胞数(P<0.05)及IL-6、IL-1β、瘦素、TNF-α、p16-INK4a蛋白和mRNA表达显著降低(P<0.01),脂联素的蛋白(P<0.05)和mRNA表达(P<0.01)显著升高;HIIT和MICT组脂肪组织p53蛋白和m RNA表达均低于SED组(P<0.01),HIIT组p-IkBα、p-JNK、p-NF-κB、p-p38MAPK、p-IkBα/p-IkBα、p-JNK/JNK、p-NF-κB/NF-κB、p-p38MAPK/p38MAPK均低于SED和MICT组(P<0.01),HIIT组p-AKT和p-AKT/AKT高于SED和MICT组(P<0.01)。相关性分析显示,p-NF-κB/NF-κB与p16-INK4a(r=0.94,P<0.01)、IL-6(r=0.77,P<0.05)、IL-1β(r=0.74,P<0.05)、TNF-α(r=0.82,P<0.01)、瘦素(r=0.92,P<0.01)呈正相关,p-AKT/AKT与p-Ik Bα/Ik Bα(r=-0.89,P<0.01)、p-JNK/JNK(r=-0.95,P<0.01)、p-p38MAPK/p38MAPK(r=-0.90,P<0.01)和p-NF-κB/NF-κB(r=-0.91,P<0.01)呈负相关。结论:HIIT比MICT能更有效地抑制内脏脂肪组织SASP分泌,该过程可能与AKT通路的激活及NF-κB、p38MAPK和JNK通路的抑制有关。Objective: To investigate the effects and mechanism of high-intensive interval training(HIIT) on senescence-associated secretory phenotype(SASP) in adipose tissue of aging rats.Methods: 36 female Sprague Dawley rats at 18 months old were selected randomly and divided into three groups: Sedentary control(SED), moderate-intensity continuous training(MICT), and HIIT groups, 12 rats per group. HIIT started with a speed of 15 m/min and lasted for 4 min. After that, the high-intensity training speed was 25 m/min, and the time was 1 min. Then, 9 cycles were successively alternated. The MICT had a speed of 17 m/min and a time of 45 min. The exercise lasted for eight months. Immunohistochemistry was used to examine the contents of betagalactosidase in visceral adipocytes. Expressions of p16-INK4a, p53, adiponectin, leptin, interleukin-6(IL-6), interleukin-1β(IL-1β), and monocyte chemoattractant protein-1(MCP-1) in visceral adipose tissue was detected by Western Blot and RT-PCR. In addition, Western Blot was performed to detect the phosphorylation levels of NF-κB, p38MAPK, AKT, IkBα, and JNK. Moreover, the protein contents of p16-INK4a, p-NF-κB, and p-IkBα were observed by immunofluorescence. Results: Compared with the SED and MICT groups, the number of β-galactosidase staining positive adipocytes, and the protein and mRNA expression of IL-6, IL-1β, leptin, TNF-α,and p16-INK4ain the HIIT group were significantly decreased(P<0.01), and the expression of adiponectin protein(P<0.05) and mRNA expression were significantly increased(P<0.01). The expression of p53 protein and m RNA in HIIT and MICT group was lower than that in SED group(P<0.01). Western blot showed that the p-IkBα, p-JNK, p-NF-κB, p-p38MAPK, p-IkBαto p-IkBα ratio, p-JNK to JNK ratio, p-NF-κB to NF-κB ratio, and p-38MAPK to p38MAPK ratio in the HIIT group were lower than those in the SED and MICT groups(P<0.01), and the p-AKT and p-AKT to AKT ratio were higher than those in the SED and MICT groups(P<0.01).Pearson correlation analysis showed that p-

关 键 词：高强度间歇训练 增龄 脂肪组织 衰老相关分泌表型 

分 类 号：G804.2[文化科学—运动人体科学]