灰葡萄孢BcPDR1与MAPK途径基因BcBMP1和BcBMP3的关系  

Relationship Between BcPDR1 Gene and MAPK Pathway Genes BcBMP1 and BcBMP3 in Botrytis cinerea

作　　者：刘晓颖 魏雅迪 李白[1] 曹宏哲 藏金萍[1] 张康 邢继红[1] 董金皋[1] LIU Xiaoying;WEI Yadi;LI Bai;CAO Hongzhe;ZANG Jinping;ZHANG Kang;XING Jihong;DONG Jingao(Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北省植物生理与分子病理学重点实验室,河北农业大学真菌毒素与植物分子病理学实验室,河北保定071000

出　　处：《华北农学报》2022年第S01期318-323,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金面上项目(32072369,31972217);中央引导地方科技发展基金项目(206Z6501G)。

摘　　要：为了确定灰葡萄孢致病基因BcPDR1与病菌MAPK途径基因BcBMP1和BcBMP3之间的关系,利用实时荧光定量PCR技术,分析BcPDR1基因突变体中BcBMP1、BcBMP3的表达情况以及BcBMP1、BcBMP3基因RNAi突变体中BcPDR1的表达情况;在突变体ΔBcpdr1背景下,构建了BcBMP1和BcBMP3过表达的菌株ΔBcpdr1/BcBMP1-OE和ΔBcpdr1/BcBMP3-OE,并对过表达菌株的表型特征和致病能力进行分析。结果发现,BcPDR1基因突变体BCt89和ΔBcpdr1中BcBMP1、BcBMP3的表达水平显著优于野生型菌株和回复菌株,BcBMP1、BcBMP3基因RNAi突变体中BcPDR1的表达水平显著低于野生型;过表达菌株ΔBcpdr1/BcBMP1-OE和ΔBcpdr1/BcBMP3-OE的菌落形态、菌丝形态、生长速率、产孢量和致病力均与突变体ΔBcpdr1表现明显的不同,而更接近野生型BC22。结果表明,灰葡萄孢BcPDR1与BcBMP1、BcBMP3的表达水平密切相关,BcPDR1负调控BcBMP1、BcBMP3的表达,而BcBMP1、BcBMP3正调控BcPDR1的表达。To clarify the relationship between BcPDR1 gene and MAPK pathway genes BcBMP1 and BcBMP3 of Botrytis cinerea.We analyzed the expression levels of BcBMP1 and BcBMP3 in mutants of BcPDR1 by Real-time PCR and the expression levels of BcPDR1 in RNAi mutants of BcBMP1 and BcBMP3.In the background of mutantΔBcpdr1,strainsΔBcpdr1/BcBMP1-OE andΔBcpdr1/BcBMP3-OE over-expressing BcBMP1 and BcBMP3 were constructed,and the phenotype and pathogenicity of the over-expressing strains were analyzed.It was found that the expression levels of BcBMP1 and BcBMP3 in BCt89 andΔBcpdr1,gene mutants of BcPDR1,were significantly better than those of the wild-type strain and the revertant strain,and the expression levels of BcPDR1 in the RNAi mutants of BcBMP1 and BcBMP3 gene were obviously lower than those of the wild-type strain.The colony morphology,hyphal morphology,growth rate,sporulation,and pathogenicity of the over-expression isolatesΔBcpdr1/BcBMP1-OE andΔBcpdr1/BcBMP3-OE were obviously different fromΔBcpdr1,and closer to the wild-type BC22.These results indicated that the BcPDR1 was closely related to the expression levels of BcBMP1 and BcBMP3 in B.cinerea.The BcPDR1 gene negatively regulates the expression of BcBMP1 and BcBMP3,while BcBMP1 and BcBMP3 genes positively regulate the expression of the BcPDR1.

关 键 词：灰葡萄孢 BcPDR1 MAPK途径基因 BcBMP1 BcBMP3 

分 类 号：S432.1[农业科学—植物病理学]