响应面法优化吸水链霉菌产雷帕霉素发酵培养基  

Optimization of Streptomyces Hygroscopicus Medium for Production of Rapamycin by Response Surface Methodology

作　　者：张晓元 张艳艳 孙晓康 刘英梅 张秀华 张林军 陈勉 刘飞 ZHANG Xiao-yuan;ZHANG Yan-yan;SUN Xiao-kang;LIU Ying-mei;ZHANG Xiu-hua;ZHANG Lin-jun;CHEN Mian;LIU Fei(Key Laboratory of Biopharmaceuticals,Shandong Academy of Pharmaceutical Sciences,Jinan 250101,China)

机构地区：[1]山东省药学科学院山东省生物药物重点实验室,山东济南250101

出　　处：《食品与药品》2023年第1期1-5,共5页Food and Drug

基　　金：山东省重大科技创新工程(编号:2021ZDSYS07);济南市高校院所创新团队项目(编号:2019GXRC038)。

摘　　要：目的优化吸水链霉菌产雷帕霉素发酵培养基,提高雷帕霉素产量。方法利用Plackett-Burman试验设计筛选出培养基中影响雷帕霉素发酵产量的显著因素,爬坡试验确定主要因素的最适范围,响应面法确定各显著因素的最优水平。结果获得最优发酵培养基配方为:葡萄糖37.60 g/L、甘露醇30 g/L、黄豆粉28.37 g/L、硫酸铵1.25 g/L、磷酸氢二钾5 g/L、磷酸二氢钾5 g/L、L-赖氨酸1.5 g/L、复合氨基酸1.83 g/L。结论在最优发酵培养基培养下,雷帕霉素发酵水平由初始182.23 mg/L提高到279.56 mg/L,提高了53.41%。Objective To optimize rapamycin-producing Streptomyces hygroscopicus medium and improve the yield of rapamycin.Methods Plackett Burman experimental design was applied to screen out the significant factors affecting the fermentation yield of rapamycin in fermentation medium.The main factors were tested through the steepest ascent method to get the optimal range.Finally,the Response Surface Methodology was used to determine the optimal level of each significant factor.Results The optimal formulation of fermentation medium was:glucose 37.60 g/L,mannitol 30 g/L,soybean powder 28.37 g/L,ammonium sulfate 1.25 g/L,dipotassium hydrogen phosphate 5 g/L,potassium dihydrogen phosphate 5 g/L,L-lysine 1.5 g/L and compound amino acid 1.83 g/L.Conclusion Under the optimal fermentation medium,the fermentation level of rapamycin can be increased from 182.23 mg/L to 279.56 mg/L,with an increase of 53.41%.

关 键 词：吸水链霉菌 雷帕霉素 响应面试验 发酵培养基 

分 类 号：Q93-335[生物学—微生物学]