茯苓皮提取物对高尿酸血症小鼠的尿酸水平和肾脏的影响  

Study on the effect of lowering uric acid and effect on the kidney of Poriae cutis in hyperuricemia mice

作　　者：赵梓童 年四辉 孙绪强 汪伟 张青 彭代银 周凌云 ZHAO Zi-tong;NIAN Si-hui;SUN Xu-qiang;WANG Wei;ZHANG Qing;PENG Dai-yin;ZHOU Ling-yun(School of Pharmacy,Wannan Medicinal College,Wuhu 241002,China;Provincial Engineering Laboratory for Screening and Revaluation of Active Compounds of Herbal Medicines in Southern Anhui,Wuhu 241002,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement,Hefei 230012,China;Anhui Province Key Laboratory of Chinese Medicinal Formula,Hefei 230012,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002 [2]皖南中草药活性成分筛选与再评价省工程实验室,安徽芜湖241002 [3]安徽中医药大学药学院,安徽合肥230012 [4]安徽道地中药质量提升协同创新中心,安徽合肥230012 [5]安徽省中药方剂重点实验室,安徽合肥230012

出　　处：《海南医学院学报》2023年第3期168-174,共7页Journal of Hainan Medical University

基　　金：安徽省高校协同项目(GXXT-2019-043);安徽省高校科学研究项目重点项目(KJ2020A0608)资助。

摘　　要：目的:研究茯苓皮提取物对高尿酸血症(HUA)小鼠的降尿酸和肾脏保护作用。方法:用醇和水溶剂提取茯苓皮活性成分,用次黄嘌呤和氧嗪酸钾建立高尿酸血症模型,70只雄性昆明小鼠随机分为空白组、模型组(500 mg/kg次黄嘌呤+250 mg/kg氧嗪酸钾)、茯苓皮醇提物(PCE)高(388 mg/kg)、低(97 mg/kg)剂量组、茯苓皮水提物(PCW)高(96 mg/kg)、低(24 mg/kg)剂量组、苯溴马隆(BEN)组(7.5 mg/kg),分别给药治疗16 d。末次给药后,收集24 h尿量(UV),测定尿液中尿酸(UUA)、肌酐(UCr);收集左右肾,观察左肾的形态及右肾的病理变化;测定血清中尿酸(SUA)、肌酐(SCr)、尿素氮(BUN);计算尿酸排泄分数(FEUA)和尿酸清除率(CUr);酶联免疫吸附测定法(ELISA)检测肾脏中阴离子转运蛋白1(OAT1)、尿酸转运体1(URAT1)、葡萄糖转运蛋白9(GLUT9)、肿瘤坏死因子α(TNF-α)和白介素6(IL-6)含量。结果:与模型组比较,PCE和PCW能增加HUA小鼠的UUA、UCr、FEUA、CUr、OAT1(P<0.05, P<0.01)、降低HUA小鼠的SUA、SCr、BUN、URAT1、GLUT9、TNF-α、IL-6(P<0.05, P<0.01);改善HUA小鼠的肾脏形态及病理变化,且优于阳性药BEN。结论:茯苓皮提取物对HUA小鼠具有降尿酸和肾脏保护作用,其作用可能与促进尿酸排泄,调节尿酸转运蛋白,降低炎症因子水平有关。Objective: To explore the pharmacodynamic and renal protective effects of Poriae cutis extract on hyperuricemia(HUA) mice.Methods: The active components from the Poriae cutis were extracted with Alcohol solvent and aqueous solvent, HUA mice were established by hypoxanthine with potassium oxonate. Totally70 male Kunming mice were randomly divided into normal, model(500 mg/kg hypoxanthine+250 mg/kg potassium oxonate), Poriae cutis ethanol(PCE) and water extract(PCW) with high and low dose(388 mg/kg and 97mg/kg), benzbromarone(BEN) groups(7.5 mg/kg), all mice were administered with corresponding medication for 16 d. After the last administration, collected the 24 h urine volume, the urine uric acid(UUA) and urine creatinine(UCr) were determined. The morphological changes of the left kidney were viewed and the pathological changes in right kidneys were observed by hematoxylin-eosin(HE) staining. The serum contents of uric acid(SUA), creatinine(SCr) and urea nitrogen(BUN) were measured. Uric acid excretion index include fractional uric acid excretion(FEUA) and uric acid clearance rate(CUr) were calculated. The contents of OAT1, URAT1, GLUT9, TNF-α and IL-6 in the kidney were detected by ELISA.Results: PCE and PCW could increase UUA, UCr, FEUA, CUr and OAT1(P < 0.05, P < 0.01), reduce SUA, SCr, BUN, URAT1, GLUT9, TNF-α and IL-6(P < 0.05, P < 0.01). The pathological and morphological changes of kidneys in HUA mice were improved and the effect is better than BEN.Conclusion: Poriae cutis extractscould lower uric acid and possessed a protective effect on the kidney of mice with HUA, which was achieved by promoting uric acid excretion, regulating the expression of uric acid transporters and reducing the level of inflammatory factors.

关 键 词：茯苓皮 高尿酸血症 尿酸 肾损伤 

分 类 号：R285.5[医药卫生—中药学]