耐力训练对小鼠力竭运动后心肌组织中circRNA-lncRNA-miRNA-mRNA表达谱的影响  

Effect of endurance training on the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues of mice after exhaustive exercise

作　　者：韩淑芳 李嘉颖 殷敏 房结 李彬 王峥 宋颖 HAN Shu-fang;LI Jia-ying;YIN Min;FANG Jie;LI Bin;WANG Zheng;SONG Ying(Department of Cardiology,Joint Logistics Support Force of People's Liberation Army 960 Hospital,Jinan 250013,China;School of Clinical Medicine,Weifang Medical College,Weifang 261000,China;Jinan's Eleventh Leaving Cadre Recuperation Center of Shandong Provincial Military Region,Jinan 250013,China)

机构地区：[1]联勤保障部队解放军第960医院心内科,山东济南250013 [2]潍坊医学院临床医学院,山东潍坊261000 [3]山东省军区济南第十一离职干部休养所,山东济南250013

出　　处：《海南医学院学报》2023年第3期175-182,共8页Journal of Hainan Medical University

基　　金：山东省医药卫生科技发展计划项目(202103011061)。

摘　　要：目的:明确耐力训练对小鼠力竭运动后心肌组织中circRNA-lncRNA-miRNA-mRNA表达谱的影响。方法:45只雄性C57BL/6小鼠随机分为对照(C)、低强度耐力训练(LSET)与高强度耐力训练(HSET)组(n=15)。对照组小鼠不进行跑台训练,LSET与HSET组小鼠分别以30%与60%力竭运动量进行跑台训练,每天一次,每周5天,训练5周后行力竭运动。取心肌组织提取总RNA,利用Illimina转录组测序分析心肌组织中circRNA-lncRNA-miRNA-mRNA表达谱。结果:LSET与HSET组小鼠力竭运动时间与路程较C组延长,且HSET组小鼠力竭运动时间与路程长于LSET组(P<0.05)。转录组测序共筛选出54个差异表达的circRNA(28个下调和26个上调),7个差异表达的lncRNA(均下调),3个差异表达的miRNA(1个下调和2个上调),99个差异表达的mRNA(81个下调和18个上调)(P<0.05)。相互作用网络分析发现ENSMUSG00000113041、MSTRG.79740、mmu-miR-374c-5p、18个下调的mRNA与3个表达上调的mRNA构成调控网络。GO功能分析发现差异表达的mRNA主要富集在初级代谢过程与大分子合成与代谢过程。KEGG通路分析发现差异表达的mRNA主要富集于补体和凝血级联反应途径、雌激素信号通路及胰高血糖素信号通路。结论:耐力训练可以改变小鼠力竭运动后心肌组织中circRNA-lncRNA-miRNA-mRNA表达谱,这些差异表达的RNA构成调控网络影响心肌细胞的合成与代谢进而参与对心肌损伤的调节。Objective: To clarify the effect of endurance training on the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues of mice after exhaustive exercise.Methods: 45 male C57BL/6 mice were randomly divided into control(C), low-strength endurance training(LSET) and high-strength endurance training(HSET) groups(n=15). The mice in the control group were not conducted to platform training. The mice in the LSET and HSET groups were conducted to platform training at 30% and 60% of exhaustive exercise once a day for 5 days a week, respectively. The exhaustion exercise was performed after 5 weeks of platform training. Total RNA was extracted from myocardial tissues, and the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues was analyzed using Illimina transcriptome sequencing.Results: The distance and time of exhaustive exercise were longer in the LSET and HSET groups than in the control group, and the distance and time of exhaustive exercise were longer in the HSET group than in the LSET group(P<0.05). A total of 54 differentially expressed circRNAs(28 down-regulated and 26 up-regulated), 7 differentially expressed lncRNAs(all down-regulated), 3 differentially expressed miRNAs(1 down-regulated and 2 up-regulated) and 99 differentially expressed mRNAs(81 down-regulated and 18 up-regulated) were identified by transcriptome sequencing(P<0.05). Interaction network analysis revealed that ENSMUSG00000113041, MSTRG. 79740, mmu-miR-374c-5p, 18 down-regulated mRNAs and 3up-regulated mRNAs formed a regulatory network. GO functional analysis revealed that the differentially expressed mRNAs were mainly enriched in primary metabolic processes and macromolecular synthesis and metabolic processes. KEGG pathway analysis revealed that the differentially expressed mRNAs were mainly enriched in complement and coagulation cascade pathways, estrogen signaling pathway and glucagon signaling pathway. Conclusion: Endurance training could alter the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tiss

关 键 词：耐力训练 力竭运动 circRNA-lncRNA-miRNA-mRNA 差异表达 调控网络 

分 类 号：G804.2[文化科学—运动人体科学]