锁阳和唐古特白刺寄生体内生细菌群落结构及功能特征  

Community Structure and Functional Characteristics of Endophytic Bacteria in Root-Parasitic Plant Cynomorium Songaricum and Its Host Nitraria Tangutorum

作　　者：张慧 纪鑫 崔晋龙[1] 王俊宏[1] 王梦亮[1] 秦雪梅[2] ZHANG Hui;JI Xin;CUI Jinlong;WANG Junhong;WANG Mengliang;QIN Xuemei(Institute of Applied Chemistry,Shanxi University,Taiyuan 030006,China;Modern Research Center for Traditional Chinese Medicine,the Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学应用化学研究所,山西太原030006 [2]山西大学中医药现代研究中心,山西大学化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《山西大学学报（自然科学版）》2022年第6期1640-1647,共8页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31670328);山西省回国留学人员科研资助项目(2020-013)。

摘　　要：以沙漠来源的锁阳-唐古特白刺(简称白刺)寄生植物体为对象,通过高通量测序分析寄生植物体内生细菌分布和组成及功能。结果表明,锁阳与寄生白刺、锁阳与非寄生白刺之间内生细菌OTU(Operational taxonomic units)组成相似性分别为11.50%和2.33%;锁阳内生细菌物种丰富度显著高于非寄生白刺(P<0.05),而锁阳和寄生白刺内生细菌丰富度差异不显著;锁阳和非寄生白刺组间UniFrac距离为0.75,而锁阳和寄生白刺组间UniFrac距离为0.35,说明锁阳与寄生白刺间的菌群结构差异显著减小。随着寄生行为的发生,寄生双方内生细菌群落组成发生显著变化,寄主白刺相对丰度较高的属为糖霉菌属和Lepidoceras;锁阳中为Lepidoceras、伯克氏菌属、鞘氨醇单胞菌属和苍白杆菌属;非寄生白刺中为Lepidoceras和伯克氏菌属。Lepidoceras和伯克氏菌属等7株菌在锁阳中的相对丰度显著高于寄生白刺(P<0.05),仅有糖霉菌属和Olivibacter在寄生白刺中相对丰度显著高于另外两种样本(P<0.05)。内生细菌功能分析表明,分解木质纤维素的主要功能基因如NADH脱氢酶(K05575)、淀粉磷酸化酶(K00688)、α-淀粉酶(K01176)和环葡聚糖水解酶(K01208)等在寄生白刺中的相对丰度显著高于锁阳(P<0.05),这对认识沙漠条件下锁阳植物的成功寄生具有重要意义。The distribution,composition and function of endophytic bacteria of Cynomorium songaricum and parasitized Nitraria tangutorum from the desert were analyzed by high-throughput sequencing.The results showed that the similarities of endophytic bacteria OTU composition between C.songaricum and parasitized N.tangutorum,and C.songaricum and non-parasitized N.tangutorum were 11.50%and 2.33%,respectively.The endophytic bacteria richness of C.songaricum was significantly higher than that of non-parasitized N.tangutorum(P<0.05),while the endophytic bacteria richness of C.songaricum and parasitized N.tangutorum was not significantly different.The UniFrac distance between the C.songaricum and non-parasitized N.tangutorum groups was 0.75,while the UniFrac distance between the C.songaricum and parasitized N.tangutorum groups was 0.35.This indicated that the difference of endophytic bacteria was significantly reduced between C.songaricum and parasitized N.tangutorum.With the occurrence of parasitic behaviors in C.songaricum,the community composition of endophytic bacteria has changed significantly.The genus with higher relative abundance in SNT were Glycomyces and Lepidoceras,in CSR were Lepidoceras,Burkholderia,Sphingomonas,and Ochrobactrum,and in NT were Lepidoceras and Burkholderia.The relative abundance of 7 strains,such as Lepidoceras and Burkholderia in C.Songaricum,was significantly higher than that of host N.tangutorum(P<0.05).Only the Glycomyces and Olivibacter were significantly more abundant in host N.tangutorum than the other two samples(P<0.05).The biological function analysis of endophytes revealed that the relative abundances of the main functional genes of lignocellulose degradation such as NADH dehydrogenase(K05575),starch phosphorylase(K00688),alpha-amylase(K01176),and cyclomaltodextrinase(K01208)in host N.tangutorum were significantly higher than those of C.songaricum(P<0.05),which were very important to occurrence of parasitic life of C.songaricum on root of N.tangutorum under desert condition.

关 键 词：寄生 内生细菌 生物学功能 寄生植物-内生细菌互作 

分 类 号：Q943.2[生物学—植物学]