水解透明质酸锌的抗痤疮功效研究  

Hydrolysed zinc hyaluronan,a novel anti-acne candidate

作　　者：陈帆 杨君 杨素萍 吴越 Fan Chen;Jun Yang;Suping Yang;Yue Wu(Department of Research and Development,Bloomage Biotechnology Co.,Ltd.,Shanghai 200000,China;BIOHYALUX Business Division,Bloomage Biotechnology Co.,Beijing 100000,China;Department of Research and Development,Bloomage Biotechnology Co.,Ltd.,Beijing 100000,China)

机构地区：[1]华熙生物科技股份有限公司上海护肤品研发中心,上海200000 [2]华熙生物科技股份有限公司个人消费品事业群,北京100000 [3]华熙生物科技股份有限公司研发中心,北京100000

出　　处：《日用化学工业（中英文）》2023年第2期165-170,共6页China Surfactant Detergent & Cosmetics

摘　　要：探究了水解透明质酸锌(HA-ZN)在控油与保湿方面的体外生物活性。通过MTT法检测了不同质量浓度的HA-ZN对SZ95和HaCaT的细胞活性的影响,利用油红染色法检测HA-ZN对SZ95细胞内的油脂含量的影响,并且利用体外抑菌实验检测了HA-ZN的抑菌作用。同时,利用免疫荧光法检测HaCaT细胞中HAS2和AQP3表达的变化。结果表明,HA-ZN可以提升锌的安全用量。0.2 mg/mL和0.225 mg/mL的HA-ZN可以抑制SZ95细胞的油脂生成。5 mg/mL的HA-ZN可以抑制痤疮丙酸杆菌和糠秕马拉色菌的生长。同时,0.225 mg/mL的HA-ZN可促进HaCaT细胞表达HAS2和AQP3。HA-ZN具有较好的控油,抑制痤疮相关的菌群的作用,同时具有一定的保湿效果,提示其具有一定的抗痤疮的功效。In this study,the effects of hydrolysed zinc hyaluronan(HA-ZN)on lipid production and skin hydration were revealed.The HaCaT and SZ95 cell lines were applied to evaluate the toxicity and beneficial effect of hydrolysed zinc hyaluronan.Pro-acne agents,arachidonic acid was used to induce the lipid production of SZ95 and the effect of hydrolysed zinc hyaluronan was detected.At the same time,in vitro antibacterial experiment against acne-related bacteria and the expression of skin hydration involved proteins were estimated.At the identical concentration of zinc ion,hydrolysed zinc hyaluronan exhibits less toxicity than zinc chloride,suggesting a higher safe dosage of zinc ion.In addition,hydrolysed zinc hyaluronan possesses the ability of alleviating lipid production in SZ95,as well as inhibiting the growth of Propionibacterium acnes and Malassezia furfur in vitro.The expression of AQP3 and HAS2 is also augmented in hydrolysed zinc hyaluronan treated group.The results indicate that hydrolysed zinc hyaluronan might be a promising drug candidate against acne vulgaris,averting the side effects of existing drugs such as dry skin.

关 键 词：痤疮 皮肤保湿 锌盐 水解透明质酸锌 

分 类 号：TQ658[化学工程—精细化工]