Development of a Handheld Nano-centrifugal Device for Visual Virus Detection  

作　　者：Zi-Rong Bi Meng-Lu Hu Yong-Zhong Jiang Er-Hu Xiong Bo-Wen Shu Si-Qi Li Han-Wei Chen Xiao-Hua Chen Xiao-Ming Zhou 

机构地区：[1]South China Normal University-Panyu Central Hospital Joint Laboratory of Basic and Translational Medical Research,Guangzhou Panyu Central Hospital,Guangzhou,511400,China [2]School of Life Sciences,South China Normal University,Guangzhou,510631,China [3]Hubei Provincial Center for Disease Control and Prevention,Wuhan,430079,China [4]Dermatology Hospital,Southern Medical University,Guangzhou,510091,China [5]Medical Imaging Institute of Panyu,Guangzhou,511400,China [6]Department of Ultrasound,Panyu Central Hospital,Guangzhou,511400,China [7]Cancer Center,Integrated Hospital of Traditional Chinese Medicine,Southern Medical University,Guangzhou,510315,China

出　　处：《Journal of Analysis and Testing》2022年第4期353-364,共12页分析检测（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(91959128,21874049,81772246);the Special Project of Science and Technology Development of Guangdong Province(2017B020207011);the Key Research and Development Plan of Hubei Province(2020BCA090);the Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC202001).

摘　　要：Gold nanoparticles(AuNPs)colorimetric assays based on distance-dependent optical characteristics have been widely employed for bioanalysis.However,this assay is not effective for visually detecting low-concentration targets due to the faint color change.Here,we developed a handheld nano-centrifugal device which could separate the crosslinked and non-crosslinked AuNPs.Results showed that the handheld nano-centrifugal device could easily reach more than 6000 r/min within 10 s simply by stretching and tightening the coiled rope in an appropriate rhythm.Further,combined with the CRISPR/Cas12a nucleic acids recognition system,a field-deployable colorimetric platform termed handheld nano-centrifugal device assisted CRISPR/Cas12a(Hand-CRISPR)has been validated.Moreover,clinical diagnostics applications for Epstein-Barr virus(EBV)and severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)detection with high sensitivity and accuracy(100%consistency with reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR)test results)have been demonstrated.Overall,the Hand-CRISPR platform showed great promise in point-of-care-test(POCT)application,expected to become a powerful supplement to the standard nucleic acid testing method in remote or poverty-stricken areas.

关 键 词：Nucleic acids assay CRISPR/Cas12a Gold nanoparticles Centrifugal device Clinical diagnostics 

分 类 号：TH776[机械工程—仪器科学与技术]