五倍子酸对D-半乳糖致衰老小鼠胸腺线粒体功能的影响  

Effect of gallic acid on thymic mitochondrial function in D-galactose treated mice*

作　　者：苏晴 杨书平 李明欣 刘莉[1] 柴玉荣[1] Su Qing;Yang Shuping;Li Mingxin;Liu Li;Chai Yurong(Department of Histology and Embryology,School of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]郑州大学基础医学院组织学与胚胎学系,郑州450001

出　　处：《解剖学杂志》2022年第6期499-502,F0002,共5页Chinese Journal of Anatomy

基　　金：河南省高等学校重点科研项目(21A310022)。

摘　　要：目的:研究五倍子酸对D-半乳糖致衰老小鼠胸腺线粒体功能的影响。方法:将昆明小鼠分为对照组、D-半乳糖致衰老模型组、五倍子酸组。H-E染色观察各组小鼠胸腺组织的结构变化;β-半乳糖苷酶染色方法检测胸腺中的衰老细胞;线粒体膜电位检测试剂盒检测线粒体膜电位变化;免疫印迹检测线粒体融合蛋白(Mfn1和Mfn2)以及动力相关蛋白1(Drp1)的表达水平;免疫组织化学方法检测Mfn2的原位表达。结果:五倍子酸能恢复胸腺正常的组织结构;与对照组比较,D-半乳糖组胸腺中的衰老细胞明显增多,线粒体膜电位明显下降,Mfn1和Mfn2表达水平无明显变化,Drp1表达水平降低;而五倍子酸组中,衰老细胞数目减少,线粒体膜电位上升,Mfn1和Mfn2表达水平无明显变化,Drp1表达水平升高,差异均有统计学意义。结论:五倍子酸对D-半乳糖致衰老小鼠的胸腺线粒体有一定保护功能,可能是通过维持线粒体融合、分裂的平衡,增加线粒体膜电位以维持线粒体正常功能。Objective:To investigate the effects of gallic acid(GA)on thymic mitochondrial function in D-galactose(D-gal)induced aging mice.Methods:Kunming mice were divided into a control group,a D-gal aging mouse model group,and a GA treatment group.The changes of thymic structure in each group were observed by H-E staining.Senescent cells in thymic tissues were detected by SA-β-Gal staining.Mitochondrial membrane potential was detected by mitochondrial membrane potential detection kit(JC-1 method).The expression levels of mitochondrial fusion proteins(Mfn1 and Mfn2)and dynamic related protein 1(Drp1)were tested by Western blotting.The situ expression of Mfn2 was further examined by immunohistochemistry.Results:After GA administration,the normal histology structure of thymus can be restored.Compared with the control group,the senescent cells in the thymus of D-gal group were significantly increased,mitochondrial membrane potential was significantly decreased,the expression levels of Mfn1 and Mfn2 were not significantly changed,and the expression level of Drp1 was decreased.In the GA group,the number of senescent cells decreased,the mitochondrial membrane potential increased,the expression levels of Mfn1 and Mfn2 did not change significantly,and the expression level of Drp1 increased.Conclusion:Gallic acid has a protective effect on thymic mitochondria function of D-galactose induced aging mice,which may be by maintaining the balance between mitochondrial fusion and division and increasing mitochondrial membrane potential to keep the normal function of mitochondria.

关 键 词：胸腺 五倍子酸 D-半乳糖 衰老 线粒体 小鼠 

分 类 号：R285.5[医药卫生—中药学]