黄芪总黄酮对肝硬化大鼠肝组织病理、代谢指标及NF-κB表达的影响  

Effects of Astragalus total flavonoids on liver pathology,metabolism indexes and NF-κB expression in rats with liver cirrhosis

作　　者：张峰 李燕 李伟华 Zhang Feng;Li Yan;Li Weihua(Jinan People’s Hospital,Jinan 271100,China)

机构地区：[1]济南市人民医院病理科,济南271100

出　　处：《解剖学杂志》2022年第6期526-531,共6页Chinese Journal of Anatomy

摘　　要：目的:探讨黄芪总黄酮对肝硬化大鼠肝组织病理、代谢指标及NF-κB表达的影响。方法:将健康大鼠随机分为健康组、肝硬化组、低浓度黄芪总黄酮组(低浓度组)、高浓度黄芪总黄酮组(高浓度组)、美他多辛组。除健康组外,给予其他各组大鼠注射40%四氯化碳花生油建立肝硬化大鼠模型。低浓度组和高浓度组给予不同浓度黄芪总黄酮灌胃;美他多辛组给予美他多辛片灌胃,用药2周。ELISA法检测各组大鼠血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、总胆红素(TBIL)、肿瘤坏死因子α(TNF-α)、白细胞介素(IL)6(IL-6)、IL-8、κ基因结合核因子(NF-κB)水平;H-E染色检测大鼠肝组织病理形态;分别采用Realtime PCR和免疫印迹检测各组大鼠肝组织中NF-κB、TNF-αmRNA及蛋白表达水平。结果:与健康组相比,肝硬化组大鼠血清中AST、ALT、TBIL、TNF-α、IL-6、IL-8水平明显升高;与肝硬化组相比,低、高浓度组,美他多辛组上述水平均有所下降;与美他多辛组相比,低、高浓度组降低明显,低、高浓度组间差异无统计学意义。H-E染色可见肝硬化组大鼠肝细胞结构完整、排列整齐;肝硬化组大鼠肝汇管区、小叶间可见大量增生胶原纤维;低、高浓度组,美他多辛组大鼠较肝硬化组肝组织纤维增生明显改善。与肝硬化组相比,低、高浓度组,美他多辛组肝组织中NF-κB、NF-κB p65、TNF-α表达水平明显下降;与美他多辛组相比,低、高浓度组肝组织中NF-κB、NF-κB p65、TNF-α表达水平降低明显,低、高浓度组相比无差异。与健康组比较,肝硬化组可见大量肝细胞凋亡;与肝硬化组相比,低、高浓度组和美他多辛组肝细胞凋亡率均明显降低;与低浓度组相比,高浓度组和美他多辛组大鼠肝细胞凋亡率降低明显,高浓度组与美他多辛组相比无差异。结论:黄芪总黄酮可以改善肝功能水平,抑制TNF-α、IL-6、IL-8和NF-κB表达,减少肝细胞Objective:To investigate the effects of Astragalus flavones on liver histomathology,metabolic indexes,and NF-κB expression in cirrhosis rats.Methods:Healthy rats were randomly divided into a healthy group,a liver cirrhosis(LC)group,a low concentration of total flavonoids group(LCT group),a high concentration of total flavonoids group(HCT group),and a metadocine(ME)group.Except for the healthy group,other groups were injected with 40%carbon tetrachloride peanut oil to establish liver cirrhosis rat models.The low concentration group and high concentration group were given different concentrations of total flavonoids by gavage.The metadocine group was given metadocine tablets by gavage.The serum levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL),TNF-α,IL-6,IL-8 and NF-κB were detected by ELISA.The pathological morphology of liver tissue was detected by H-E staining.Realtime PCR and Western blotting were used to detect the mRNA and protein expressions of NF-κB and TNF-αin liver tissues of rats in each group.Results:Compared with the healthy group,the serum levels of AST,ALT,TBIL,TNF-α,IL-6 and IL-8 in LC group were significantly increased.Compared with the LC group,the above levels in the LCT group,the HCT group and the ME group were decreased.Compared with the ME group,the LCT group and the HCT group were significantly decreased,and there was no difference between the LCT group and the HCT group.H-E staining showed that the structure of liver cells in the cirrhosis group was complete and orderly.A large number of proliferative collagen fibers could be seen in hepatic portal area and interlobular area in the LC group.Compared with the LC group,liver tissue fiber proliferation was significantly improved in the LCT group,the HCT group and the ME group.Compared with the LC group,the expression levels of NF-κB,NF-κB p65,and TNF-αin liver tissues of the LCT group,the HCT group,and the ME group were significantly decreased.Compared with the ME group,the expression levels

关 键 词：黄芪总黄酮 肝硬化 组织病理 代谢指标 κ基因结合核因子 

分 类 号：R285.5[医药卫生—中药学]