sTIM-3及其配体Gal-9、HMGB1与2型糖尿病并发冠心病的相关性研究  

Correlation research between sTIM-3 and its ligands Gal-9,HMGB1 in patients with type 2 diabetes complicated with coronary heart disease

作　　者：刘艳秋[1] 范海迪 侯海燕 孙健 林宁[3] LIU Yanqiu;FAN Haidi;HOU Haiyan;SUN Jian;LIN Ning(Department of Clinical Laboratory,Branch Hospital of Huai'an First People's Hospital,Huai'an 223002,China;Department of Clinical Laboratory,Qinhuai District Center for Disease Control and Prevention;Department of Clinical Laboratory,Huai'an First Hospital Affiliated to Nanjing Medical University)

机构地区：[1]淮安市第一人民医院分院检验科,223002 [2]南京市秦淮区疾病预防控制中心检验科 [3]南京医科大学附属淮安第一医院检验科

出　　处：《天津医药》2023年第3期294-298,共5页Tianjin Medical Journal

基　　金：江苏省淮安市自然科学研究计划基金资助项目(HAB202137)。

摘　　要：目的探讨血清中可溶性T细胞免疫球蛋白及黏蛋白分子-3(sTIM-3)、半乳糖凝集素-9(Gal-9)、高迁移率族蛋白B1(HMGB1)水平与2型糖尿病(T2DM)并发冠心病(CHD)的关系。方法选取T2DM组患者50例、T2DM并发CHD组(T2DM+CHD组)患者52例,同期健康体检人员(Con组)48例。采用酶联免疫吸附试验(ELISA)检测3组血清中s TIM-3、Gal-9及HMGB1的水平。Spearman法分析血清sTIM-3、Gal-9、HMGB1、空腹血糖(FBG)、超敏C-反应蛋白(hs-CRP)间的相关性;使用受试者工作特征(ROC)曲线分析sTIM-3、Gal-9及两者联合检测对T2DM并发CHD的诊断能力;采用Logistic回归分析T2DM并发CHD的危险因素。结果与Con组相比,T2DM组和T2DM+CHD组sTIM-3、Gal-9、HMGB1升高(P<0.05);与T2DM组相比,T2DM+CHD组sTIM-3和Gal-9升高(P<0.05),HMGB1差异无统计学意义。FBG、hs-CRP与sTIM-3、Gal-9、HMGB1呈正相关,sTIM-3与Gal-9、HMGB1,Gal-9与HMGB1呈正相关(P<0.05)。ROC曲线分析结果显示血清sTIM-3和Gal-9联合诊断T2DM并发CHD曲线下面积为0.812(95%CI:0.743~0.882)。Logistic回归分析显示体质量指数、sTIM-3、Gal-9升高是T2DM并发CHD的危险因素。结论血清s TIM-3、Gal-9升高是T2DM并发CHD的危险因素。Objective To investigate the relationship between serum soluble T cell immunoglobulin and mucin domain-3(sTIM-3),galectin-9(Gal-9)and high mobility group protein B1(HMGB1)levels in patients with type 2 diabetes mellitus(T2DM)complicated with coronary heart disease(CHD).Methods Fifty patients with T2DM were used as the T2DM group,and fifty-two patients with T2DM complicated with CHD were selected as the T2DM+CHD group.Forty-eight healthy physical examination subjects were selected as the control group(Con group).Serum levels of sTIM-3,Gal-9 and HMGB1 were detected by enzyme-linked immunosorbent assay(ELISA).Spearman correlation analysis was used to analyze the correlation between serum sTIM-3,Gal-9,HMGB1,fasting blood glucose(FBG)and high-sensitivity C-reactive protein(hs-CRP).Receiver operating characteristic curve(ROC)was used to analyse the diagnostic capability of sTIM-3,Gal-9 and their combination in T2DM complicated with CHD.Logistic regression was used to investigate the risk factors of T2DM complicated with CHD.Results Compared with the Con group,sTIM-3,Gal-9 and HMGB1 were significantly increased in the T2DM group and the T2DM+CHD group(P<0.05).Compared with the T2DM group,sTIM-3 and Gal-9 were significantly increased in the T2DM+CHD group(P<0.05),and there was no significant difference in HMGB1.FBG and hsCRP were positively correlated with sTIM-3,Gal-9 and HMG B1,sTIM-3 was positively correlated with Gal-9 and HMGB1,and Gal-9 was positively correlated with HMGB1(P<0.05).ROC curve analysis showed that the area under the curve of serum sTIM-3 and Gal-9 in the diagnosis of T2DM complicated with CHD was 0.812(95%CI:0.743-0.882).Logistic regression analysis showed that increased body mass index,sTIM-3 and Gal-9 were risk factors for T2DM complicated with CHD.Conclusion Increased serum levels of sTIM-3 and Gal-9 are risk factors for T2DM complicated with CHD.

关 键 词：糖尿病 2型 冠状动脉疾病 半乳糖凝集素类 高迁移率族蛋白质类 可溶性T细胞免疫球蛋白及黏蛋白分子-3 

分 类 号：R392.6[医药卫生—免疫学]