福建灵芝6个主要三萜类成分的含量测定及其在溯源中的应用  

Determination of Six Main Triterpenoids from Ganoderma Lucidum of Fujian and its Application in Traceability

作　　者：张基荣 张颖 蒋昆霞[1,2] 陈素芳 黄黎娜 吴长辉 许文 林羽[1,2] Zhang Jirong(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou Fujian 350122)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学科技创新与转化中心,福建福州350122 [3]福建仙芝楼科技有限公司,福建南平350002

出　　处：《黑龙江医药》2023年第1期4-10,共7页Heilongjiang Medicine journal

基　　金：国家重点研发计划(编号:2019YFC1710505),课题题目:全链条质量可追溯体系构建。

摘　　要：目的:本研究旨在建立超高效液相色谱(UPLC)测定福建灵芝中6个主要三萜类成分(灵芝酸I、灵芝酸G、灵芝酸B、灵芝酸LM 2、灵芝酸A、灵芝酸C 1)的含量,并对比福建灵芝与其他产区灵芝的含量差异,为福建灵芝在质量溯源中的应用提供实验依据。方法:采用Agilent ZORBAX-SB-C 18(2.1×150mm,1.8μm)色谱柱,以乙腈(A)-0.1%甲酸水(B)为流动相,梯度洗脱,流速0.25mL·min^(-1),柱温25℃,检测波长254nm,进样量5μL。结果:灵芝酸I、灵芝酸G、灵芝酸B、灵芝酸LM 2、灵芝酸A、灵芝酸C 1线性范围分别为0.7440~37.20、0.9750~48.75、1.200~60.00、1.325~66.25、3.900~195.0、1.350~67.50μg·mL^(-1);福建灵芝中上述6个三萜类成分含量分别为0.0323~0.0404、0.0750~0.1008、0.2702~0.3804、0.1028~0.1257、0.4730~0.5817、0.2554~0.3039mg·g^(^(-1))。化学计量学聚类分析,当欧式距离为76时,福建灵芝可以溯源区分;主成分分析可将灵芝样品可以分为吉林、安徽、浙江、福建4个产区,进一步的正交偏最小二乘(OPLS-DA)法分析显示,灵芝酸LM 2、灵芝酸C 1和灵芝酸I的VIP均大于1,是福建灵芝区别于其他产区的差异标志物。结论:该方法可同时测定福建灵芝样品中6个三萜类成分的含量,并应用于福建灵芝的质量追溯,可为福建灵芝的质量追溯提供一种新的技术手段。Objective:To establish the ultra performance liquid chromatography(UPLC)for the determination of 6 main triterpenoids(Ganoderic acid I,Ganoderic acid G,Ganoderic acid B,Ganoderic acid LM 2,Ganoderic acid A,Ganoderic acid C 1)in Ganoderma lucidum of Fujian,and to compare the content difference between Fujian and other production areas,which provided an experimental basis for the application to Fujian Ganoderma lucidum traceability.Methods:The chromatographic separation was performed on an Agilent ZORBAX-SB-C 18(2.1×150mm,1.8μm)with acetonitrile(A)-0.1%formic acid aqueous solution(B)as mobile phases.The flow rate was 0.25mL·min^(-1) and the column temperature was 25℃.The detection wavelength was set at 254 nm and 5μL of the sample was injected for analysis.Results:The linear ranges of Ganoderic acid I,Ganoderic acid G,Ganoderic acid B,Ganoderic acid LM 2,Ganoderic acid A and Ganoderic acid C 1 were 0.7440~37.20,0.9750~48.75,1.200~60.00,1.325~66.25,3.900~195.0,1.350~67.50μg·mL^(-1).The content ranges of the above 6 triterpenoids in different regions of Fujian Ganoderma lucidum were 0.0323~0.0404,0.0750~0.1008,0.2702~0.3804,0.1028~0.1257,0.4730~0.5817,0.2554~0.3039mg·g^(-1).Stoichiometric cluster analysis,when the Euclidean distance was 76,Fujian Ganoderma lucidum could be traced to distinguish.Principal component analysis could divide samples into Jilin,Anhui,Zhejiang and Fujian.The results of orthogonal partial least square(OPLS-DA)method showed that the VIP of Ganoderic acid LM 2,Ganoderic acid C 1 and Ganoderic acid I were all greater than 1,which were the difference markers of Fujian Ganoderma acid from other producing areas.Conclusion:UPLC method could be used for simultaneous determination of 6 main triterpenoids in Fujian Ganoderma lucidum,and could be applied to the quality traceability of Fujian Ganoderma lucidum,which providing a new technique for the quaiity traceability of Fujian Ganoderma lucidum.

关 键 词：灵芝 超高效液相色谱法 聚类分析 主成分分析 正交偏最小二乘法 追溯 

分 类 号：R927.2[医药卫生—药学]