小麦NF-Y家族基因TaNF-YA1介导植株耐旱功能研究  

Functional characterization of wheat TaNF-YA1, a NF-Y transcription factor gene in mediating drought resistance

作　　者：解亚蒙 赵晓蕾 白菁华 孙丽颖 马自飞 路文静[1] 肖凯[2] XIE Yameng;ZHAO Xiaolei;BAI Jinghua;SUN Liying;MA Zifei;LU Wenjing;XIAO Kai(College of Life Sciences,Hebei Agricultural University,Baoding 071001,China;College of Agronomy,Hebei Agricultural University,Baoding 071001,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学农学院,河北保定071001

出　　处：《河北农业大学学报》2023年第1期1-9,共9页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31872869,32071935);大中学生科技创新能力培育专项(22E50043D);河北农业大学科研发展基金计划项目(JY2021022)。

摘　　要：NF-Y型转录因子在调控植物生长发育和介导植株抵御非生物胁迫中发挥着重要功能。本研究分析了小麦NF-YA家族转录因子基因TaNF-YA1分子特征、应答干旱表达模式和介导植株耐旱生物学功能。TaNF-YA1在核苷酸序列上与一粒小麦TmNF-YA9高度同源。干旱处理下,根系和叶片中该基因表达丰度随处理进程不断增大,处理后24 h达到峰值,48 h保持稳定;将处理后48 h植株转入正常生长后根叶中该基因转录本随恢复处理进程不断减少。与野生型对照(WT)相比,干旱处理下,正义表达TaNF-YA1烟草株系(Sen 1和Sen 2)生长势增强,植株个体增大,根鲜重提高,干质量增加,光合碳能力提升,细胞抗氧化酶活性增强。该正义株系干旱胁迫下气孔关闭速率加快,离体叶片持水率增大。干旱处理下Sen 1根系发育基因(NtPIN1和NtPIN4)和抗氧化酶基因(NtSOD1.1、NtCAT2.2和NtPOD4.1)表达水平较WT显著增高。与WT相比,反义表达TaNF-YA1烟草株系(Anti 1和Anti 2)干旱处理下植株生长势、干物质积累、生理参数(光合生理参数、细胞保护酶活性、气孔关闭速率、叶片持水率)和基因表达特征与Sen 1和Sen 2呈相反趋势。这些结果表明,TaNF-YA1通过在转录水平上对干旱胁迫产生应答,调控植株干旱适应相关生理生化过程,改善植株根系发育、光合物质生产和细胞活性氧稳态,进而增强植株抵御干旱胁迫能力。NF-Y-type transcription factors play an important role in regulating plant growth and development and mediating plant resistance to abiotic stress. This study investigated the molecular characteristics, expression pattern in response to drought stress, and biological function in mediating plant drought tolerance of a transcription factor gene TaNF-YA1 belonging to the wheat NF-YA family. TaNF-YA1sequence was highly similar to TmNF-YA9 of Triticum monococcumin. Under drought treatment, the expression of TaNF-YA1 in roots and leaves increased continuously and peaked at 24 h after treatment, which remained stable until 48 h after treatment. In addition, the gene transcripts in roots and leaves decreased gradually when plants were recovered to normal condition after 48 h of drought treatment.Compared with the wild-type(WT) control, the growth vigor of the tobacco lines overexpressing sense TaNFYA1(Sen 1 and Sen 2) was enhanced under drought treatment, showing increasedplant size, fresh and dry weight of root. Moreover, these transgenic lines displayed enhancedability of photosynthetic carbon assimilationactivity of cellular antioxidant enzymes, and increased stomatal closure rate andwater holding capacity in the isolated leaves after drought stress. The expression levels of the antioxidant enzyme genes(NtSOD1.1, NtCAT2.2 and NtPOD4.1)and root development-associated genes(NtPIN1 and NtPIN4) were significantly increased in Sen 1 under drought treatment in comparison with the WT plants. Consistently, the tobacco lines expressing antisense TaNF-YA1(Anti 1 and Anti 2) showed decrease in plant growth, dry matter accumulation, physiological parameters(photosynthetic physiological parameters, cell protective enzyme activity, stomatal closure rate, water holding capacity of leaf) and drought related gene expressions. This study suggested that TaNF-YA1 promotes root development, photosynthetic assimilate production and homeostasis of cellular reactive oxygen species(ROS), and responds to drought stress to regulate the physiolo

关 键 词：小麦 转录因子 TaNF-YA1基因 干旱胁迫 遗传转化 功能分析 

分 类 号：S512[农业科学—作物学]