Rs-198液体有机菌肥制备及其促生性能研究  

Preparation of Rs-198 liquid organic bacterial fertilizer and its growth-promoting performance

作　　者：朱双喜 武占省 何艳慧 ZHU Shuangxi;WU Zhansheng;HE Yanhui(School of Environmental and Chemical Engineering/Xi’an Key Laboratory of Textile Chemical Engineering Auxiliaries,Xi’an Polytechnic University,Xi’an 710048,China)

机构地区：[1]西安工程大学环境与化学工程学院/西安市纺织化工助剂重点实验室,陕西西安710048

出　　处：《西安工程大学学报》2023年第1期71-76,共6页Journal of Xi’an Polytechnic University

基　　金：陕西省人社厅留学人员择优资助计划(2020018)。

摘　　要：为简化液体有机菌肥的制备工艺,将液体有机肥和植物根际促生菌Pseudomonas putida Rs-198复配,分析Rs-198液体有机菌肥的制备工艺、稳定性及其对萝卜幼苗促生能力的影响。结果表明:液体有机肥的最佳稀释倍数为5倍;Rs-198在稀释液体有机肥中的最佳培养条件为30℃、140 r/min、装液量100 mL;摇瓶发酵36 h时,菌体密度最大可达4.6×10^(9)CFU/mL;制备的Rs-198液体有机菌肥室温储存28 d后有效活菌数为2×10^(7)CFU/mL;膨润土作为其最佳营养助剂,有效活菌数提高了60.2%。液体有机菌肥可显著提高萝卜幼苗生物量,其中株高、鲜重和干重分别增加19.6%、22.2%和17.6%,萝卜叶片中可溶性糖和可溶性蛋白含量分别提高49.4%和12.4%,Rs-198液体有机菌肥应用前景良好。In order to explore the preparation process of liquid organic bacterial fertilizer,the liquid organic fertilizer and plant rhizosphere growth-promoting bacteria Pseudomonas putida Rs-198 were compounded,and the preparation technology and stability of Rs-198 liquid organic bacterial fertilizer and its growth promotion effect on the radish seedlings were studied.The results showed that the optimal dilution ratio of liquid organic fertilizer was 5 times;the optimal culture conditions of Rs-198 in diluted liquid organic fertilizer were 30℃,140 r/min,and the medium volume was 100 mL;the maximum bacterial density can reach 4.6×10~9 CFU/mL;the prepared Rs-198 liquid organic fertilizer was stored at room temperature for 28 days,and the effective viable count was 2×10~7 CFU/mL.In addition,bentonite as its best nutritional supplement can increase the number of effective viable bacteria by 60.2%.The pot experiment showed that the liquid organic bacterial fertilizer could significantly increase the biomass of radish seedlings in which the plant height,fresh weight and dry weight increased by 19.6%,22.2%and 17.6%,respectively.The content of soluble sugar and soluble protein increased by 49.4%and 12.4%,respectively.Therefore,Rs-198 liquid organic bacterial fertilizer has a good application prospect.

关 键 词：液体有机肥 Pseudomonas putida Rs-198 液体有机菌肥 萝卜幼苗 生物量 

分 类 号：TQ424[化学工程] X703[环境科学与工程—环境工程]