牛miR-206潜在靶基因及其组织表达模式分析  

Analysis of Potential Target Genes and Tissue Expression Patterns of Cattle(Bos taurus)miR-206

作　　者：张岩峰 丁燕玲 李鹏[1] 李浩宁 史远刚[1] 康晓龙[1] ZHANG Yan-Feng;DING Yan-Ling;LI Peng;LI Hao-Ning;SHI Yuan-Gang;KANG Xiao-Long(School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《农业生物技术学报》2023年第1期98-104,共7页Journal of Agricultural Biotechnology

基　　金：宁夏回族自治区区重大科技项目(2017BY078);宁夏自然科学基金(2020AAC03075)。

摘　　要：miR-206是前期课题组通过全转录组测序筛选出的高低剩余采食量肉牛(Bos taurus)中差异显著的小RNA(microRNAs,miRNAs)之一,为探究miR-206对牛肌肉生长发育的调节作用,本研究以’秦川牛’miR-206为研究对象,分析了miR-206在各物种间的亲缘关系,通过TargetScan、miRWalk等在线软件预测了牛miR-206潜在靶基因并对靶基因进行GO和KEGG富集分析,通过qPCR方法检测牛各组织中miR-206的表达规律。结果表明,牛miR-206与山羊(Capra hircus)的亲缘关系最近,其次是马(Equus caballus),与爪蟾(Xenopus laevis)的亲缘关系最远;miR-206的潜在靶基因中与肌肉发育相关的有组蛋白去乙酰酶4(histone deacetylase 4,HDAC4)、热休克蛋白家族D成员1(heat shock protein family d member 1,HSPD1)、纤维连接蛋白1(fibronectin,FN1)等;功能富集分析发现,预测的靶基因显著富集于MAPK、PI3K-AKT和TNF等与肌肉发育相关的信号通路。qPCR结果显示,miR-206在成年牛腿肌和背最长肌中的表达量最高且极显著高于其他组织(P<0.01),其次是肝脏,在十二指肠及皮下脂肪中的表达量最低;与初生犊牛相比,成年牛皮下脂肪中miR-206表达量极显著下调(P<0.01),背最长肌和腿肌中表达量极显著上调(P<0.01)。本研究结果将为进一步探究miR-206在牛肌肉生长发育过程中的作用提供参考。miR-206 is one of the miRNAs previously screened by our group by whole-transcriptome sequencing which showed significant differences in high and low residual feed intake in beef cattle(Bos taurus).Cattle miR-206 was used as a study target to analyze the affinity of miR-206 among species,and the potential target genes of bovine miR-206 were predicted by online software such as TargetScan and miRWalk.GO and KEGG enrichment analysis of the target genes were performed.The expression pattern of miR-206 in cattle tissues was detected by qPCR.The results showed that cattle miR-206 was most closely related to goat(Capra hircus),followed by horse(Equus caballus),and the most distantly related to Xenopus laevis.Among the potential target genes of miR-206 related to muscle development were histone deacetylase 4(HDAC4),heat shock protein family D member 1(HSPD1),and fibronectin(FN1).The functional enrichment analysis revealed that the predicted target genes were significantly enriched in MAPK,PI3K-AKT and TNF,which were signaling pathways related to muscle development.qPCR results showed that miR-206 expression was the highest in the leg and longest dorsal muscles of adult cattle and was extremely significant higher than that in other tissues(P<0.01),followed by liver,and the lowest was in the duodenum and subcutaneous.Compared with newborn calves,miR-206 expression was extremely significant down-regulated in subcutaneous fat and up-regulated in dorsal longest muscle and leg muscle(P<0.01)in adult cattle.The results of this study will provide a reference for further investigation of the role of miR-206 in the growth and development of cattle muscle.

关 键 词：miR-206 组织表达 靶基因预测 qPCR 富集通路 

分 类 号：S823.8[农业科学—畜牧学]