Genome-wide analysis of AP2/ERF superfamily in Isatis indigotica  

作　　者：Liang Xiao Jun-ze Ren Qing Li Bin Yang Zhen-jiang Liu Rui-bing Chen Lei Zhang 

机构地区：[1]Department of Pharmaceutical Botany,School of Pharmacy,Naval Medical University,Shanghai 200433,China [2]Medical School of Nantong University,Nantong 226001,Jiangsu Province,China [3]Department of Pharmacy,Changzheng Hospital,Naval Medical University,Shanghai 200433,China [4]School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China

出　　处：《Journal of Integrative Medicine》2023年第1期77-88,共12页结合医学学报（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(No.32000231,31970316,32170274,31770329 and 32070327);Program of Shanghai Academic Research Leader(19XD1405000);Shanghai Sail Program(19YF1459300)。

摘　　要：Objective AP2/ERF(APETALA2/ethylene-responsive factor)superfamily is one of the largest gene families in plants and has been reported to participate in various biological processes,such as the regulation of biosynthesis of active lignan.However,few studies have investigated the genome-wide role of the AP2/ERF superfamily in Isatis indigotica.This study establishes a complete picture of the AP2/ERF superfamily in I.indigotica and contributes valuable information for further functional characterization of IiAP2/ERF genes and supports further metabolic engineering.Methods To identify the IiAP2/ERF superfamily genes,the AP2/ERF sequences from Arabidopsis thaliana and Brassica rapa were used as query sequences in the basic local alignment search tool.Bioinformatic analyses were conducted to investigate the protein structure,motif composition,chromosome location,phylogenetic relationship,and interaction network of the IiAP2/ERF superfamily genes.The accuracy of omics data was verified by quantitative polymerase chain reaction and heatmap analyses.Results One hundred and twenty-six putative IiAP2/ERF genes in total were identified from the I.indigotica genome database in this study.By sequence alignment and phylogenetic analysis,the IiAP2/ERF genes were classified into 5 groups including AP2,ERF,DREB(dehydration-responsive element-binding factor),Soloist and RAV(related to abscisic acid insensitive 3/viviparous 1)subfamilies.Among which,122 members were unevenly distributed across seven chromosomes.Sequence alignment showed that I.indigotica and A.thaliana had 30 pairs of orthologous genes,and we constructed their interaction network.The comprehensive analysis of gene expression pattern in different tissues suggested that these genes may play a significant role in organ growth and development of I.indigotica.Members that may regulate lignan biosynthesis in roots were also preliminarily identified.Ribonucleic acid sequencing analysis revealed that the expression of 76 IiAP2/ERF genes were up-or down-regulated under salt

关 键 词：Isatis indigotica AP2/ERF Genomean alysis Expression pattern Lignan biosynthesis 

分 类 号：S567.2[农业科学—中草药栽培]