醒神解郁汤对卒中后抑郁模型大鼠proBDNF/mBDNF平衡及海马神经损伤的影响  

Effects of Xingshen Jieyu decoction on proBDNF/mBDNF balance and hippocampal nerve injury in post-stroke depression model rats

作　　者：祁丽丽[1] 侯臻臻 童碧薇 陈祥 刘德志 张佳[3] 周一心[3,7] 韩振翔 Qi Lili;Hou Zhenzhen;Tong Biwei;Chen Xiang;Liu Dezhi;Zhang Jia;Zhou Yixin;Han Zhenxiang(Department of Emergency,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai,200080,P.R.China;Department of Biological and Chemical Engineering,Jining Vocational and Technical College,Jining,Shandong,27200,P.R.China;Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200137,P.R.China;Community Health Service Center of Gucun Town,Baoshan District,Shanghai,201907,P.R.China;Shanghai University of Traditional Chinese Medicine,Shanghai,201203,P.R.China;Department of Neurology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,201203,P.R.China;Center for Disease Control and Prevention of Pudong New District,Shanghai,200136,P.R.China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院急诊科,上海200080 [2]山东济宁职业技术学院生物与化学工程系,山东济宁272000 [3]上海中医药大学附属第七人民医院,上海200137 [4]上海顾村镇社区卫生服务中心,上海201906 [5]上海中医药大学,上海201203 [6]上海中医药大学附属曙光医院神经内科,上海201203 [7]上海市浦东新区疾病预防控制中心,上海200136

出　　处：《老年医学与保健》2023年第1期132-138,共7页Geriatrics & Health Care

基　　金：上海市浦东新区民生科研专项(PKJ2021-Y03);上海市科委医学创新研究专项(21Y11921000);上海市科委自然科学基金(21ZR1449300);上海市浦东新区卫生健康委国家中医药发展综合改革试验区建设项目(PDZY-2021-0404);上海市浦东新区名中医培养计划(PWRzm2020-08);上海市浦东新区领先人才培养计划(PWRl2021-05);上海市浦东新区卫生系统优秀青年医学人才(PWRq2020-34);国家中医药管理局中医药创新骨干人才培训项目(2019)。

摘　　要：目的研究醒神解郁汤在卒中后抑郁(PSD)大鼠模型中,对脑组织中proBDNF/mBDNF平衡以及血清中IL-17表达的影响,探究其影响神经损伤可能的作用机制。方法65只SD大鼠随机分为假手术组、卒中后抑郁组、醒神解郁汤低/高剂量组以及舍曲林组。假手术组在大脑中动脉缺血再灌注(MCAO)术中只分离动脉不做阻断。卒中后抑郁组在MCAO术后施加慢性温和性不可预见性应激压力(CUMS)以建立PSD模型;PSD建模成功后,分别以醒神解郁汤低剂量(18.72 g·kg^(-1)·d^(-1))、高剂量(37.44 g·kg^(-1)·d^(-1))及舍曲林(5.142 g·kg^(-1)·d^(-1))灌胃,连续14 d。记录大鼠强迫游泳静止不动时间,利用透射电镜观察大鼠海马组织结构,分别采用Wester blotting、ELISA检测大鼠脑组织proBDNF/mBDNF水平及血清IL-17水平。结果醒神解郁汤可明显改善卒中后抑郁模型大鼠海马组织神经损伤。与假手术组相比,卒中后抑郁组强迫游泳静止不动时间、IL-17水平、proBDNF/mBDNF比值均显著增加,差异有统计学意义(P<0.05)。醒神解郁汤低、高剂量组与卒中后抑郁组相比,强迫游泳静止不动时间、IL-17水平、proBDNF/mBDNF比值均明显下降,差异有统计学意义(P<0.05)。结论醒神解郁汤可通过调控大鼠proBDNF/mBDNF比值及血清IL-17表达,改善卒中后抑郁大鼠的神经损伤。Objective To study the effects of Xingshen Jieyu decoction on proBDNF/mBDNF balance in brain tissue and the expression of serum IL-17 in post-stroke depression(PSD)model rats,and explore the possible mechanism of its influence on nerve injury.Methods 65 SD rats were divided randomly into four groups:sham-operation group,PSD group,low-doses group and high-doses group of Xingshen Jieyu decoction and sertraline group.In the sham-operation group,only the artery was isolated during middle cerebral artery ischemia-reperfusion(MCAO)without blocking.The rats in other groups were exposed to chronic unpredictable mild stress after MCAO operation to establish PSD model.After PSD modeling was successful,low dose of Xingshen Jieyu decoction(18.72 g·kg^(-1)·d^(-1)),high dose of Xingshen Jieyu decoction(37.44 g·kg^(-1)·d^(-1))or sertraline(5.142 g·kg^(-1)·d^(-1))were administered by gavage for consecutive 14 days,respectively.The immobile time of forced swimming test was recorded.The structure of hippocampus of rat was observed under transmission electron microscope.The levels of proBDNF/mBDNF in brain tissue and IL-17 in serum of rats were measured by Wester blotting and ELISA,respectively.Results Xingshen Jieyu decoction could alleviate nerve injury in hippocampus of post-stroke depression model rats.Compared with the sham-operation group,the immobile time of forced swimming test,IL-17 level and proBDNF/mBDNF ratio in the PSD group increased significantly,with statistical significance(P<0.05).Compared with the PSD group,the immobile time of forced swimming test,IL-17 level and proBDNF/mBDNF ratio in the low-dose and high-dose Xingshen Jieyu decoction group decreased significantly,with statistical significance(P<0.05).Conclusion Xingshen Jieyu decoction can alleviate the nerve injury in hippocampus by regulating the ratio of proBDNF/mBDNF and the expression of serum IL-17 in PSD rats.

关 键 词：卒中后抑郁 醒神解郁汤 白介素-17 proBDNF/mBDNF 

分 类 号：R285.5[医药卫生—中药学]