苁蓉舒痉颗粒对帕金森病模型大鼠延髓的保护作用  

Protective Effect of Congrong Shujing Granules on Medulla Oblongata of Rats with Parkinson’s Disease

作　　者：林瑶[1] 唐岚芳 李茜羽 黄佩珍 许茜[1] 王林[1] 袁明洲 蔡晶[3] LIN Yao;TANG Lanfang;LI Qianyu;HUANG Peizhen;XU Qian;WANG Lin;YUAN Mingzhou;CAI Jing(College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Xiuyuan Clinical Medical College,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China)

机构地区：[1]福建中医药大学中西医结合学院,福建福州350122 [2]福建中医药大学中西医结合研究院,福建福州350122 [3]福建中医药大学修园临床医学院,福建福州350122

出　　处：《福建中医药》2023年第1期10-15,共6页Fujian Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81804156,81904263);国家自然科学基金面上项目(82074507);福建省自然科学基金项目(2018J01882)。

摘　　要：目的观察苁蓉舒痉颗粒对帕金森病(PD)模型大鼠延髓的保护作用及机制。方法将50只健康SD大鼠随机分为对照组10只和造模组40只。造模组大鼠于颈背部皮下注射鱼藤酮葵花油乳化液来建立PD模型,对照组大鼠颈背部皮下注射等体积葵花油。将30只造模成功的大鼠再次随机分为模型组、苁蓉舒痉组、美多芭组各10只,分别予相应药液进行灌胃,连续干预14 d。分别于造模后和干预第7、14天进行网格实验和斜板实验;免疫组化法检测中脑黑质区TH阳性细胞数;TUNEL染色法观察延髓细胞凋亡情况;应用透射电镜观察延髓细胞线粒体形态;Western blot法检测大鼠中脑黑质区α-突触核蛋白(α-synuclein)和延髓B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、激活型半胱氨酸蛋白酶3(cleaved caspase-3)、过氧化物酶体增殖物激活受体γ(PPARγ)、过氧化物酶体增殖物激活受体-γ共激活因子-1α(PGC-1α)的蛋白表达。结果与对照组比较,模型组大鼠造模后和干预7、14 d后移动潜伏期明显缩短和斜板倾斜角度明显减小(P<0.05);中脑黑质区TH阳性细胞数明显减少(P<0.05);延髓细胞凋亡率明显增加(P<0.05),线粒体肿胀;中脑黑质区α-synuclein和延髓cleaved caspase-3、Bax蛋白表达明显提高(P<0.05),大鼠延髓Bcl-2、PPARγ和PGC-1α蛋白明显降低(P<0.05)。与模型组比较,苁蓉舒痉组和美多芭组大鼠干预7、14 d后移动潜伏期明显延长和斜板倾斜角度明显增大(P<0.05);黑质区TH阳性细胞数明显增加(P<0.05);延髓细胞凋亡率降低(P<0.05),线粒体结构接近正常;中脑黑质区α-synuclein和延髓cleaved caspase-3、Bax蛋白表达明显降低(P<0.05),延髓Bcl-2、PPARγ和PGC-1α蛋白明显提高(P<0.05)。结论苁蓉舒痉颗粒能减轻PD模型大鼠延髓神经细胞凋亡,这可能与改善PD模型大鼠延髓神经细胞线粒体形态,增加线粒体合成蛋白PPARγ及PGC-1α表达有关。Objective:To observe the protective effect and mechanism of Congrong Shujing(CRSJ)granules on medulla oblongata in rats with Parkinson’s disease(PD).Methods:Fifty SD rats were randomly divided into control group(n=10)and modeling group(n=40).The PD model was induced by subcutaneous injection of rotenone sunflower oil emulsion at the rat’s nape,and rats in the control group were injected with the same volume sunflower oil.Thirty rats with successful modeling were randomly divided into model group,CRSJ group and madopar group,with 10 rats in each group.Each group was respectively given corresponding drug solution by gavage for 14 consecutive days.Grid experiment and inclined plate experiment were carried out respectively after modeling and on the 7th and 14th day of intervention.The number of tyrosine hydroxylase(TH)positive cells in the substantia nigra of the midbrain was detected by immunohistochemistry;TUNEL staining was used to observe the apoptosis of medulla oblongata cells;the morphology of mitochondria in medulla oblongata cells was observed by transmission electron microscopy;the protein expression ofα-synuclein in the substantia nigra of the midbrain and peroxisome proliferator-activated receptor-γ(PPARγ),peroxisome proliferator-activated receptor gamma coactivator 1-α(PGC-1α),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X(Bax)and cleaved caspase-3in the substantia nigra of the midbrain was detected by Western blot.Results:Compared with the control group,the latency of movementin the model group significantly shortened after modeling and on the 7th and 14th day of intervention,and the angles of inclined planesignificantly decreased(P<0.05);the number of TH positive cells in the substantia nigra of the midbrain significantly decreased(P<0.05);the apoptosis rate of medulla oblongata cells significantly increased and the mitochondria swelled(P<0.05);the protein expression ofα-synuclein in the midbrain substantia nigra and the protein expression of cleaved caspase-3 and Bax in the medulla oblongata si

关 键 词：帕金森病 苁蓉舒痉颗粒 细胞凋亡 线粒体 PPARΓ PGC-1Α 

分 类 号：R285.5[医药卫生—中药学]