肝郁对围绝经期综合征大鼠认知功能及脑组织氧化应激的影响  

Effect of Liver Depression on Cognitive Function and Oxidative Stress of Brain Tissue in Rats with Perimenopausal Syndrome

作　　者：沈建英[1,2] 梁文娜 张凌媛[1,2] 丁珊珊 康洁[1,2] 赵田 陈燕[1] 刘圣洋 杨敏[1,2] 龚琳 SHEN Jianying;LIANG Wenna;ZHANG Lingyuan;DING Shanshan;KANG Jie;ZHAO Tian;CHEN Yan;LIU Shengyang;YANG Min;GONG Lin(Research Base of Traditional Chinese Medicine Syndrome,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Fujian Key Laboratory of Traditional Chinese Medicine Health State,Fuzhou,Fujian 350122,China)

机构地区：[1]福建中医药大学中医证研究基地,福建福州350122 [2]福建省中医健康状态辨识重点实验室,福建福州350122

出　　处：《福建中医药》2023年第1期20-24,共5页Fujian Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82174248,81774209);福建省自然科学基金项目(2022J01362);福建中医药大学基础类学科科研提升计划项目(XJC2022012);福建中医药大学创新创业训练计划项目(201710393022,201810393010,201910393022)。

摘　　要：目的观察围绝经期综合征大鼠在肝气郁结的状态下其认知行为及抗氧化应激能力的改变。方法通过生理周期和旷场行为筛选出12月龄围绝经期综合征SD大鼠32只,采用随机数字表法按体质量分层将大鼠分为对照组(n=12)和模型组(n=20)。模型组采用慢性束缚应激结合孤养法建立围绝经期综合征肝郁大鼠模型,最后成模大鼠12只;对照组以每笼4只进行群养。采用Morris水迷宫的定位航行实验及空间探索实验分别检测2组大鼠的记忆保持能力及空间学习能力。检测2组大鼠前额叶皮质及海马超氧化物歧化酶(SOD)的活力、丙二醛(MDA)含量评价其抗氧化应激能力。结果造模前,2组大鼠皮毛色泽光亮,精神状况良好,活动反应灵敏;造模后,对照组大鼠体征无明显变化,模型组大鼠则表现出食欲下降、粪便稀软、反应迟钝、精神萎靡、焦躁不安、偶有嘶叫等现象。与对照组比较,模型组造模后大鼠血浆的ACTH和CORT含量明显升高(P<0.01);糖水消耗率明显下降(P<0.01);旷场实验水平得分和垂直得分也明显下降(P<0.01);平均逃避潜伏期明显延长(P<0.01);空间探索实验中在第三象限的平均停留时间明显缩短(P<0.01);海马及前额叶皮质组织SOD活力明显下降(P<0.01),MDA含量明显上升(P<0.01)。结论肝郁会导致模型组SD大鼠认知功能减退及抗氧化应激能力下降,提示肝郁可能会加速围绝经期综合征大鼠脑组织衰老的进程。Objective:To observe the changes in cognitive behavior and antioxidant stress ability of rats with perimenopausal syndrome in the state of liver stagnation.Methods:Thirty-two 12-month-old SD rats with perimenopausal syndrome were selected through physiological cycle and open field test,which were divided into control group(n=12)and model group(n=20)by random number table according to body mass.In the model group,chronic restraint stress combined with solitary rearing were used to establish the rat model of liver depression of perimenopausal syndrome,and 12 rats were finally established.The control group was fed with 4 animals per cage.The memory retention ability and spatial learning ability of rats were tested by Morris water maze positioning navigation training and spatial probetraining.The contents of adrenocorticotropic hormone(ACTH)and cortisol(CORT)in rat plasma were detected.The activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in the prefrontal cortex and hippocampus of rats were measured.Results:Before the establishment of the model,the rats in the two groups had bright fur,good mental state and sensitive activity response.After the establishment of the model,the physical signs of the rats in the control group did not change significantly,while the rats in the model group showed decreased appetite,weak feces,slow response,lethargy,restlessness,and occasional hissing.Compared with the control group,the contents of ACTH and CORT in the plasma of rats in the model group significantly increased(P<0.01);sugar water consumption rate significantly decreased(P<0.01),and the horizontal and vertical scores of open field test also significantly decreased(P<0.01);the average escape latency significantly prolonged(P<0.01);the average residence time in the third quadrant significantly shortened(P<0.01);the activity of SOD in hippocampus and prefrontal cortex significantly decreased(P<0.01),and the content of MDA significantly increased(P<0.01).Conclusion:Liver depression can lead to the decli

关 键 词：围绝经期综合征 肝郁 脑衰老 认知行为 氧化应激 

分 类 号：R271.116[医药卫生—中医妇科学] R-332[医药卫生—中医学]