基于指纹图谱与多指标含量测定的黔产香榧假种皮质量控制研究  

Study on quality control of Torreya grandis aril based on fingerprint and multi-index content determination

作　　者：田鑫 张艳 余明 曹泮相 张建永 段灿灿 Tian Xin;Zhang Yan;Yu Ming;Cao Panxiang;Zhang Jianyong;Duan Cancan(Department of Pharmaceutical Analysis,School of Pharmacy,Zunyi Medical University,Zunyi Guizhou 563099,China;Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education,Zunyi Medical University,Zunyi Guizhou 563099,China;Guizhou Wuchuan Wannianfeng Agricultural Development Co.,Ltd,Zunyi Guizhou 564300,China)

机构地区：[1]遵义医科大学药学院药物分析教研室,贵州遵义563099 [2]遵义医科大学基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室,贵州遵义563099 [3]贵州务川万年峰农业开发有限公司,贵州遵义564300

出　　处：《遵义医科大学学报》2023年第2期189-198,共10页Journal of Zunyi Medical University

基　　金：遵义市优秀青年科技创新人才培养项目(NO:遵优青科[2021]3)。

摘　　要：目的建立黔产香榧假种皮的高效液相色谱(HPLC)指纹图谱及多指标含量测定方法,为黔产香榧假种皮的资源利用提供科学依据。方法采用Agilent Eclipse Plus C 18色谱柱(4.6 mm×150 mm,5μm);以甲醇(A)-0.06%醋酸-水溶液(B)为流动相,梯度洗脱;流速1.0 mL/min;检测波长是230、255、270、330 nm;柱温30℃;进样量5μL。结果18批香榧假种皮指纹图谱相似度均大于0.995,共确定18个共有峰,指认了苯甲酸和金松双黄酮。对样品中的苯甲酸、异鼠李素、芹菜素、金松双黄酮和异银杏素5种化学成分进行含量测定和方法学考察,包括专属性、线性、精密度、重复性和稳定性,均符合要求;平均回收率在96.98%~101.08%,RSD≤1.57%。香榧假种皮中该5种化学成分的含量分别为(56.49~110.91、183.14~418.64、69.81~104.91、105.59~197.17、270.36~400.22)μg/g。结论本研究所建立的HPLC指纹图谱和多指标含量测定的方法准确可行,可作为黔产香榧假种皮质量评价的一种有效手段。Objective Establishment of fingerprinting and multi-component content determination methods by high performance liquid chromatography(HPLC)of the Torreya grandis aril,providing the scientific basis for resource utilization of Torreya grandis aril.Methods Agilent Eclipse Plus C 18 column(4.6 mm×150 mm,5μm)was used.With methanol(A)-0.06%acetic acid-water solution(B)as mobile phase,gradient elution was performed.The flow rate was 1.0 ml/min.The detection wavelengths were 230,255,270 and 330 nm.The column temperature was 30℃.The injection volume was 5μl.Results The similarity of 18 batches of Torreya grandis aril fingerprints were all greater than 0.995.A total of 18 common peaks were identified,and benzoic acid and sciadopitysin were identified.The methodological investigations were carried out on the determination of benzoic acid,isorhamnetin,apigenin,sciadopitysin,and isoginkgetin in the samples,including specificity,linearity,precision,repeatability,and stability,and all met the requirements.The average recoveries ranged from 96.98%to 101.08%,RSD≤1.57%.The contents of these five chemical components in Torreya grandis aril were(56.49-110.91,183.14-418.64,69.81-104.91,105.59-197.17,270.36-400.22)μg/g,respectively.Conclusion The method established by this research is accurate and feasible,and can be used as an effective means for quality evaluation of Torreya grandis aril.

关 键 词：香榧假种皮 指纹图谱 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学]