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作 者:洪滔[1] 张鸿 黄辉 熊锦泽 钟毅 李庆媛 江兰兰 伍紫文 谢林榕 刘志勇[1] HONG Tao;ZHANG Hong;HUANG Hui;XIONG Jinze;ZHONG Yi;LI Qingyuan;JIANG Lanlan;WU Ziwen;XIE Linrong;LIU Zhiyong(Jiangxi University of Chinese Medicine,330004,Nanchang,PRC;Jiangzhong Pharmaceutical Co.Ltd.of China Resources Group,330004,Nanchang,PRC)
机构地区:[1]江西中医药大学,南昌330004 [2]华润江中制药集团有限责任公司,南昌330004
出 处:《江西科学》2023年第1期50-56,61,共8页Jiangxi Science
基 金:国家大学生创新创业项目(202210412043S);江西中医药大学大学生创新创业项目(202210412145)。
摘 要:研究了生物菌肥对生姜的增产作用和土壤细菌群落的结构和多样性的影响。于4月份将生物菌肥作为底肥与传统施肥方式一样施入垄内姜种之间,11月采收生姜,称重,采用水蒸气蒸馏法提取2种肥料培养的生姜挥发油,用气相色谱-质谱联用(GC-MS)技术测定其化学成分获得离子流图。采集施用生物菌肥和施用传统肥料的九山生姜田土壤共10份样品,采用16S rDNA序列分析生姜土壤田土壤细菌群落结果和多样性。施用生物菌肥的生姜长势优于传统肥料,收获的生姜块大且色泽金黄,生姜增产37.2%,土壤细菌种类和丰度增加。在属水平上,与对照组相比,施用生物菌肥生姜土壤较传统肥组鞘脂单胞菌属Sphinogomonas、鞘氨醇单胞菌属Sphingomonas、诺卡氏菌属Nocardioides差异较大。结论:生物菌肥显著增加了生姜的产量,影响了土壤细菌的丰度和组成分布,放线菌门和变形菌门是优势菌群。To investigate the effects of biobacterial fertilizer on the yield increase of ginger and the structure and diversity of soil bacterial community. In April, the biobacterial fertilizer was applied to the ginger seeds in the ridge as a bottom fertilizer and the traditional fertilization method, and the ginger was harvested in November, weighed, and the two fertilizer-cultured ginger volatile oils were extracted by water vapor distillation, and the chemical composition was determined by gas chromatography-mass spectrometry(GC-MS) technology to obtain ion flow maps. A total of 10 samples were collected from Jiushan Ginger Field soil with biobacterial fertilizer application and traditional fertilizer application, and the results and diversity of soil bacterial communities in ginger soil were analyzed by 16S rDNA sequence. The growth of ginger under biobacterial fertilizer was better than that of traditional fertilizer, the harvested ginger was large and golden in color, the yield of ginger was increased by 37.2%, and the species and abundance of soil bacteria increased. At the genus level, compared with the control group, the biobacterial fertilizer and the traditional aficial sphingomone genus Sphinogomonas, norank_f__Vicinamibacterace genus, Sphingomonas and Nocardioides were quite different. Conclusion: Biobacterial fertilizer significantly increased the yield of ginger, which affected the abundance and composition distribution of soil bacteria, and the actinomycetes and proteobacteria phylum were the dominant flora.
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