基于Luminex技术对比分析激活后的脐血和成人富血小板血浆研究  

Bioactive ingredients in activated platelet-rich plasma derived from cord blood and adult blood via Luminex technology: a comparative study

作　　者：马秉正 赵而海 王进[3] 毛桂杰[3] 赵加美 徐荣[3] 方源[3] 左振柏 MA Bingzheng;ZHAO Erhai;WANG Jin;MAO Guije;ZHAO Jiamei;XU Rong;FANG Yuan;ZUO Zhenbo(Department of Joint Surgery,Municipal Hospital of Qingdao,Qingdao 266100,China;Huikang Hospital of Qingdao;Affliated Hospital of Qingdao University)

机构地区：[1]青岛市立医院关节外科,山东青岛266100 [2]青岛市慧康医院 [3]青岛大学附属医院关节外科

出　　处：《中国输血杂志》2023年第1期11-15,共5页Chinese Journal of Blood Transfusion

基　　金：山东省医学会脐带血科研专项基金-脐血复合凝胶在慢性创面的应用研究(YXH2020ZX010)。

摘　　要：目的 应用Luminex技术探讨脐血与成人富血小板血浆激活后释放的活性成分差异,探究反映其炎症调节能力的合理指标以指导制备。方法 选择健康成人63份(31男∶32女)外周血与61份脐血(30男∶31女)制备PRP,基于Luminex技术比较PRP激活后的20种活性因子的差异,对比超敏C反应蛋白(hs-CRP)、降钙素原(PCT)、金属蛋白酶(MMPs)/抑制剂(TIMPs)比值与10种炎症调节因子间的相关性。结果 脐血PRP诸因子、蛋白酶无性别差异,有10种利于组织修复的活性成分高于成人,10种细胞因子/蛋白酶低于成人;成人PRP除IGF-1和HGF无性别差异外,多数成分女多于男(P<0.05),MMPs/TIMPs与炎症相关的诸因子间密切关联,可反映PRP的炎症调节能力。结论 脐血不受年龄/性别影响,低免疫活性使脐血PRP释出多种以MMPs/TIMPs为靶点的活性因子,构成完善的降低MMPs/TIMPs配比的炎症调节网络。Objective To compare the bioactive ingredients in activated platelet-rich plasma(PRP) from cord blood and adult blood, explore its reasonable indicators reflecting the inflammatory regulation ability, in order to guide the preparation. Methods PRP was prepared and activated from 63 healthy adults(31 males, 32 females) and 61 neonates(30 males, 31 females), and 20 cytokines were measured using Luminex technology for assessing the age-and sex-based bioactive differences of PRP. High-sensitivity C-reactive protein(hs-CRP),procalcitonin and MMPs/TIMPs from each sample were measured for their correlations with the 10 inflammation-related cytokines. Results The activated cord blood PRP released 10 growth factors and chemokines more than the adult blood PRP, whereas IGF-1,HGF and 8 pro-inflammatory cytokines lower than the latter. Most cytokines of adult PRP were more in females than in males(P<0.05), except for IGF-1 and HGF, which showed no difference by gender. Compared with hs-CRP and PCT, MMPs/TIMPs ratio was more closely related with the inflammation-related cytokines, which can reflect the inflammatory regulation of PRP. Conclusion Due to the lower immunocompetence and no age or gender disturbance, cord blood PRP has the rational MMPs/TIMPs ratio and more cytokines which promote the inflammation and wound healing.

关 键 词：富血小板血浆 Luminex技术 超敏C反应蛋白 降钙素原 基质金属蛋白酶 金属蛋白酶抑制剂 

分 类 号：R457.14[医药卫生—治疗学] R331.143[医药卫生—临床医学]