大连地区献血者丁型肝炎病毒抗体的筛查  

Screening of hepatitis D virus in blood donors in Dalian

作　　者：刘丹[1] 王丹[1] 刘笑春 李春祥[1] 臧亮[1] 邓雪莲[1] LIU Dan;WANG Dan;LIU Xiaochun;LI Chunxiang;ZANG Liang;DENG Xuelian(Dalian Blood Center,Dalian 116001,China)

机构地区：[1]大连市血液中心,辽宁大连116001

出　　处：《中国输血杂志》2023年第1期59-63,共5页Chinese Journal of Blood Transfusion

基　　金：辽宁省自然科学基金指导计划项目(2019-ZD-0882)。

摘　　要：目的 了解丁型肝炎病毒在大连地区无偿献血人群中的流行情况。方法 挑选本实验室HBV筛查反应性剩余血浆标本,其确证结果需符合以下4种情况:1)HBsAg+&HBV DNA+;2)HBsAg+&HBV DNA-;3)HBsAg-&HBV DNA+;4)NAT-yield可疑。同时随机选取抗-HBc+或抗-HBs+的筛查合格血浆标本。所有标本进行HDV IgG初筛,结果为反应性者采用另1种HDV IgG试剂复测并检测HDV IgM。HDV IgG双试剂反应性者确证为HDV IgG+。结果 在1 344份血液筛查不合格标本(HBsAg+&HBV DNA+507份,HBsAg+&HBV DNA-33份,HBsAg-&HBV DNA+477份,可疑标本327份)和766份筛查合格标本(抗-HBc+397份、单独抗-HBs+369份)中未检出HDV抗体阳性标本。结论 大连地区献血前初筛合格的献血者HDV感染率极低。但对于HBV高流行地区,仍旧不能够忽视OBI合并感染HDV可能给血液安全带来的风险。Objective To investigate the prevalence of hepatitis D virus in Dalian blood donors. Methods The samples reactive to HBV in blood screening were selected with the following confirmed results: 1)HBsAg+&HBV DNA+;2)HBsAg+& HBV DNA-;3)HBsAg-&HBV DNA+;4)NAT-yield uncertain. Qualified samples in routine blood screening were additionally tested with anti-HBc+ and anti-HBs+. All samples selected were tested HDV IgG further. Initial reactive samples would be tested by another HDV IgG assay and HDV IgM assay. HDV IgG positive was confirmed when samples were reactive to two HDV IgG assays. Results None HDV antibodies were detected among 1 344 unqualified samples(507 HBsAg+&HBV DNA+, 33 HBsAg+&HBV DNA-,477 HBsAg-&HBV DNA+ and 327 NAT-yield uncertain samples) or 766 qualified samples(397 anti-HBc+ and 369 anti-HBs samples) in blood screening. Conclusion The prevalence of HDV infections among Dalian blood donors eligible in pre-donation screening seemed extremely low. However, for areas with high HBV prevalence, the risk of blood safety caused by OBI co-infection with HDV should not be ignored.

关 键 词：献血人群 丁型肝炎病毒 HDV抗体筛查 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学] R373.2