肽能神经元对颈部急性炎性痛模型大鼠穴位敏化的调节作用  

Role of peptidergic neurons in modulating acupoint sensitization caused by neck acute inflammatory pain in rats

作　　者：袁伟 岳江鑫 王强 吴楠 李永丰 杨晓航 乔海法 Wei YUAN;Jiang-xin YUE;Qiang WANG;Nan WU;Yong-feng LI;Xiao-hang YANG;Hai-fa QIAO(College of Acupuncture&Tuina,Shaanxi University of Chinese Medicine,Xixian New Area,Shaanxi,712046,China;Shaanxi Key Laboratory of Acupuncture&Medicine,Xixian New Area,Shaanxi,712046,China;Xianyang Key Laboratory of Neurobiology(Acupuncture),Xianyang,Shaanxi,712046,China;College of Medical Technology,Shaanxi University of Chinese Medicine,Xixian New Area,Shaanxi,712046,China)

机构地区：[1]陕西中医药大学针灸推拿学院,中国陕西省西城新区7120046 [2]陕西省针药结合重点实验室,中国陕西省西城新区7120046 [3]咸阳市神经生物学(针灸)重点实验室,中国陕西省西城新区7120046 [4]陕西中医药大学医学技术学院,中国陕西省西城新区7120046

出　　处：《World Journal of Acupuncture-Moxibustion》2023年第1期44-50,共7页世界针灸杂志（英文版）

基　　金：Supported by the National Key Research and Development Program of China:2018YFC1704606。

摘　　要：Objective:Pain is a health problem frequently reported in the clinical and general populations.Acupoint therapy is one of the most effective ways to treat pain;however,its mechanism remains unclear.Therefore,this study aimed to explore the relationship between distal acupoints,dorsal root ganglion(DRG)neurons,and spinal cord dorsal horn(SDH) neurons in neck acute inflammatory pain(NAIP).Methods:NAIP model rats were used to explore the relationship between acupoint sensitization and pain.Out of fourteen rats,ten rats were grouped into control and NAIP groups,five rats in control and five rats in NAIP.Mustard oil was subcutaneously injected on one side between the C4 and C7 vertebrae of the neck to establish an NAIP model.Evans blue(EB) was injected through the tail vein to detect sensitized acupoints after NAIP modeling.EB exudation in the body,Liè qūe(列缺 LU7),and Língdào(灵道 HT4) were evaluated.An immunofluorescence assay was conducted to detect the expression of calcitonin generelated peptide(CGRP),isolectin B4(IB4),and c-Fos in the dorsal root ganglion(DRG)and spinal cord dorsal medullae spinalis(CDMS).Four rats were used for the retrograde labeling of neurons of the LU7 region to the DRG and CDMS using CTB-488 and CTB-555 microinjection.Results:NAIP was shown to lead to oozing and pain sensitization in the LU7 and HT4 and increased the proportion of c-Fos^(+)/CGRP^(+)and c-Fos^(+)/IB4^(+)cells in both the DRG and CDMS.CTB-488 and CTB-555injected into the LU7 and sensitization point areas were observed in the DRG and CDMS regions of NAIP rats.Conclusions:Our study revealed that NAIP could lead to oozing and pain sensitization in the LU7 and HT4regions and that the pain point around LU7 might result from the transfer of peptidergic(CGRP-positive)and non-peptidergic(IB4-positive) neurons in the DRG and CDMS.

关 键 词：Neck acute inflammatory pain Acupoint sensitization Peptidergic neurons Lieque(LU7) Dorsal root ganglion Spinal cornu dorsale medullae spinalis 

分 类 号：R245[医药卫生—针灸推拿学]