S100-A8蛋白在脊柱结核患者外周血中的表达及临床意义  

Expression and clinical significance of S100-A8 protein in peripheral blood of patients with spinal tuberculosis

作　　者：任智博 费骏 娄才立 刘建 吉金科 张旭[4] 俞梦楚 牛宁奎[1] REN Zhibo;FEI Jun;LOU Caili(Department of Orthopedics,General Hospital of Ningxia Medical University,Yinchuan,750004,China;School of Clinical Medicine,Ningxia Medical University,Yinchuan,750004,China;不详)

机构地区：[1]宁夏医科大学总医院骨科,银川市750004 [2]宁夏医科大学临床医学院,银川市750004 [3]浙江大学医学院附属杭州市胸科医院骨科,杭州市310003 [4]宁夏医科大学总医院医学科学研究院,银川市750004

出　　处：《中国脊柱脊髓杂志》2023年第1期62-69,共8页Chinese Journal of Spine and Spinal Cord

基　　金：国家自然科学基金项目(编号:81860395);国家自然科学基金项目(编号:82260436);2022年自治区重点研发计划项目(编号:2022BEG03099);杭州市农业和社会发展科研主动设计项目(编号:20180417A04)。

摘　　要：目的:分析S100-A8蛋白在脊柱结核患者外周血中的表达与临床诊断的相关性,评价其作为脊柱结核诊断标志物的价值.方法:收集2018年9月~2021年6月宁夏医科大学总医院脊柱骨科收治的脊柱结核患者100例作为观察组,健康体检者30例作为对照组.观察组男性50例,女性50例,年龄49.5±16.3岁;对照组男性12例,女性18例,年龄53.4±9.7岁.两组各选取3例样本进行蛋白质组学检测并筛选差异蛋白,结合脊柱结核信号通路,确认目标蛋白.选取50例观察组与30例对照组,运用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)测定并比较两组血清目标蛋白表达水平.收集观察组临床病历资料,包括:年龄、性别、病程、实验室检查、细菌培养、T-SPOT、抗酸染色、病理学及影像学资料,收集对照组实验室检查并与观察组进行比较;采用T-test分析目标蛋白在观察组各项临床资料之中的表达差异;通过线性回归及双变量斯皮尔曼相关性分析目标蛋白与观察组临床资料的相关性;受试者工作特征曲线(receiver operating characteristic,ROC)曲线分析目标蛋白联合STB常用诊断指标的价值.结果:S100-A8蛋白具有诊断脊柱结核的价值(AUC=0.931,P<0.001),在观察组外周血中表达水平(59.04±19.37ng/ml)显著高于对照组(43.16±10.07ng/ml)(P<0.05).观察组CRP及ESR显著高于对照组(P<0.05),两组间白细胞、中性粒细胞、淋巴细胞无统计学差异(P>0.05).观察组中、细菌学培养阳性16例,阴性84例;T-SPOT阳性80例,阴性20例;抗酸染色阳性19例,阴性81例;病理学阳性73例,阴性27例;影像学诊断阳性76例,阴性24例.S100-A8蛋白在观察组的临床分组(年龄、性别、病程、白细胞、淋巴细胞、中性粒细胞)无统计学意义(P>0.05),在影像学诊断中差异显著(P<0.05),在ESR、CRP、T-SPOT、病理学结果、抗酸染色结果、细菌学检测中差异极显著(P<0.01).线性回归分析表明S100-A8蛋白表�Objectives:To analyze the correlation between the expression of S100-A8 protein in the peripheral blood of patients with spinal tuberculosis(STB)and its clinical diagnosis,and to evaluate its value as a diagnostic marker for spinal tuberculosis.Methods:100 patients with STB who were admitted to the General Hospital of Ningxia Medical University from September 2018 to June 2021 were included as the observation group and 30 healthy people were enrolled as the control group.The observation group included 50 males and 50 females,with an average of 49.5±16.3 years;the control group consisted of 12 males and 18 females,with an average age of 53.4±9.7 years.Three samples from each group were selected for proteomic detection and differential proteins were screened,and target protein was confirmed after considering spinal tuberculosis signal pathway.50 cases of observation group and 30 cases of control group were selected,Enzyme-linked immunosorbent assay(ELISA)was used to measure and compare the expressions of target proteins in the two groups.The clinical data were collected,including age,gender,course of disease,laboratory examination,bacterial culture,T-SPOT,acid-fast stain(AFS),pathology,and imaging data,and the laboratory test data of the observation group were also collected and compared with the control group.The expression difference of target protein in various clinical data of STB patients was analyzed;the correlation between target protein and clinical data of STB patients was analyzed by linear regression and Spearman correlation;ROC curve analysis was used to analyze the value of target protein combining traditional STB diagnostic indicators.Results:S100-A8 protein had the value in the diagnosis of STB was(AUC=0.931,P<0.001),and the expression of S100-A8 protein in peripheral blood of patients of observation group(59.04±19.37ng/ml)was significantly higher than that of the control group(43.16±10.07ng/ml)(P<0.05).The measurement results of C-reactive protein(CRP)and ESR in the peripheral blood of spinal tu

关 键 词：脊柱结核 蛋白质组学 S100-A8 生物标志物 

分 类 号：R529.2[医药卫生—内科学]