昆明地区2019年HIV/HCV共感染患者HCV核衣壳蛋白基因分型  

Genotyping of nucleocapsid protein gene of HCV in HIV/HCV co-infected patients in Kunming in 2019

作　　者：朱燕涛 刘俊仪 张米 张念 李健健 杨壁珲 亢丽娟 李雄君 刘家法 王佳丽 ZHU Yan-tao;LIU Jun-yi;ZHANG Mi;ZHANG Nian;LI Jian-jian;YANG Bi-hun;KANG Li-juan;LI Xiong-jun;LIU Jia-fa;WANG Jia-li(Yunnan Provincial Hospital of Infectious Diseases,Kunming,Yunnan650301,China;School of Public Health,Kunming Medical University,Kunming,Yunnan 650224,China;School of PublicHealth,Dali University,Dali,Yunnan 671003,China)

机构地区：[1]云南省传染病医院,云南昆明650301 [2]昆明医科大学公共卫生学院,云南昆明650224 [3]大理大学公共卫生学院,云南大理6710031

出　　处：《中国热带医学》2023年第1期16-21,共6页China Tropical Medicine

基　　金：国家自然科学基金(No.81960605);云南省教育厅项目(No.2019J1305);云南省科技厅-昆明医科大学应用基础研究联合专项面上项目(No.202101AY070001-223,No.202201AY070001-208);云南省科技厅重大专项(No.202102AA310005);昆明医科大学艾滋病共感染传染性疾病诊疗科技创新团(No.CXTD202111);云南省高层次卫生技术人才培养经费资助项目(No.H-2018050)。

摘　　要：目的 基于丙型肝炎病毒(hepatitis C virus, HCV)核衣壳蛋白(nucleocapsid protein)基因序列了解昆明地区人类免疫缺陷病毒(human immunodeficiency virus, HIV)/HCV共感染患者中HCV基因型及基因亚型流行情况。方法 2019年3—8月在云南省传染病医院收集临床诊断为HIV/HCV共感染住院且户籍地为昆明所辖14个县级市、区及县的患者血清,从血清中提取病毒RNA,反转录合成第一链cDNA,采用HCV核衣壳蛋白基因特异性引物进行巢式PCR扩增。结果 共收集到临床诊断疑似HIV/HCV患者样本64例,采用HCV核衣壳蛋白基因特异性引物扩增,其中17例样品扩增阳性。序列分析结果显示,有9例病例的序列与HCV 3b基因亚型序列位于同一个进化分支,核苷酸同源性为93.3%~95.2%;5例病例的序列与HCV 1b基因亚型序列位于同一个进化分支,核苷酸同源性为96.8%~97.6%;1例病例序列与HCV 3a基因亚型序列位于同一个进化分支,核苷酸同源性为95.2%;1例病例序列与HCV 6n基因亚型序列位于同一个进化分支,核苷酸同源性为97.9%;1例病例与HCV 6u基因亚型序列位于同一个进化分支,核苷酸同源性为98.4%。结论 昆明地区存在着HCV 1b、HCV 3a、HCV 3b、HCV 6n和HCV 6u等多个基因型/基因亚型HCV流行,以HCV 3b为主要流行的基因亚型。Objective To investigate the distribution characteristics of HCV genotypes and subtypes in patients with HIV(human immunodeficiency virus, HIV)/HCV co-infection in Kunming based on the nucleocapsid protein gene sequence of HCV(hepatitis C virus).Methods Serum was collected from HIV/HCV co-infected patients with household registration in 14county-level cities, districts and counties under the jurisdiction of Kunming, who admitted to Yunnan Provincial Infectious Disease Hospital from March to August 2019. The viral RNA was extracted from the serum, reverse transcribed to synthesize cDNA, and the HCV nucleocapsid protein gene-specific primers were used for nested PCR amplification. The positive amplification products were sequenced, bioinformatics software such as DNAstar and MEGAX were used for sequence analysis.Results A total of 64 samples from co-infected patients with clinical diagnosis of suspected HIV/HCV were collected and amplified by HCV nucleocapsid protein gene-specific primers, of which 17 samples were amplified positively. The results of sequence analysis showed that the sequences of 9 cases were located in the same evolutionary branch as the HCV 3b subtype sequence, and the nucleotide homology was 93.3%-95.2%;the sequences of 5 cases were located in the same evolutionary branch as the HCV 1b subtype sequence, and the nucleotide homology was 96.8%-97.6%;the sequence of one case and the subtype sequence of HCV 3a gene were located in the same evolutionary branch, and the nucleotide homology was 95.2%;the sequence of one case and HCV 6n gene subtype sequence were located in the same evolutionary branch, and the nucleotide homology was 97.9%;One case was located in the same evolutionary branch as the HCV 6u gene subtype sequence, and the nucleotide homology was 98.4%.Conclusions HCV 1b, HCV 3a, HCV 3b, HCV 6n and HCV 6u genotypes or subtypes of HCV are prevalent in Kunming, and HCV 3b is the most prevalent genotype.

关 键 词：丙型肝炎病毒 昆明地区 基因亚型 核衣壳蛋白 

分 类 号：R512.63[医药卫生—内科学]