阿尔茨海默病患者血清Ang、TIMP3水平与β-淀粉样蛋白沉积及认知功能的关系  

Relationship between serum Ang,TIMP3 levels andβ-amyloid deposition in patients with Alzheimer′s disease and cognitive function

作　　者：周慧敏 陈静[1] 王御林 欧诒丹 钟纯正 ZHOU Huimin;CHEN Jing;WANG Yulin;OU Yidan;ZHONG Chunzheng(Department of Neurology,Danzhou People′s Hospital Affiliated to Hainan Medical University,Danzhou,Hainan 571700,China)

机构地区：[1]海南医学院附属儋州市人民医院神经内科,海南儋州571700

出　　处：《国际检验医学杂志》2023年第4期416-420,共5页International Journal of Laboratory Medicine

基　　金：海南省卫生健康行业科研项目(20A190026)。

摘　　要：目的探讨阿尔茨海默病(AD)患者血清血管抑制素(Ang)、组织金属蛋白酶抑制剂3(TIMP3)水平与β-淀粉样蛋白(Aβ)沉积及认知功能的关系。方法将2020年1月至2022年5月于该院治疗的143例AD患者纳入研究作为AD组,另选取同期55例体检健康者作为对照组。采用酶联免疫吸附法检测血清Ang、TIMP3水平。采用Pearson/Spearman相关分析AD患者血清Ang、TIMP3水平与Aβ1-40、Aβ1-42、总Tau(t-Tau)、磷酸化Tau181(p-Tau181)水平和简易精神状态检查表(MMSE)评分的相关性,绘制受试者工作特征(ROC)曲线分析血清Ang、TIMP3水平对AD的诊断价值。结果AD组血清Ang、TIMP3水平均低于对照组,差异有统计学意义(P<0.05)。Pearson/Spearman相关分析显示:AD患者血清Ang、TIMP3水平与Aβ1-40水平(r=-0.446、-0.465,P<0.05)及MMSE评分(r=-0.558、-0.607,P<0.05)均呈负相关,与Aβ1-42水平呈正相关(r=0.443、0.437,P<0.05),与t-Tau、p-Tau181水平无明显的相关性(P>0.05)。ROC曲线分析显示,血清Ang、TIMP3水平单项及联合用于AD诊断的曲线下面积(AUC)分别为0.798、0.793、0.901,灵敏度分别为96.50%、67.13%、74.13%,特异度分别为56.36%、80.00%、90.91%。血清Ang、TIMP3水平联合用于AD诊断的AUC大于二者单项用于AD诊断(P<0.05)。结论血清Ang、TIMP3水平降低与AD患者认知功能下降和Aβ沉积有关,可作为AD诊断的辅助指标,而且血清Ang、TIMP3水平联合检测有助于提升对AD的诊断效能。Objective To investigate the relationship between serum angiostatin(Ang),tissue inhibitor of metalloproteinase 3(TIMP3)levels andβ-amyloid(Aβ)deposition and cognitive function in patients with Alzheimer′s disease(AD).Methods A total of 143 AD patients treated in the hospital from January 2020 to May 2022 were enrolled in the study as the AD group,and 55 healthy people who underwent healthy examination in the hospital during the same period were enrolled as the control group.The serum levels of Ang and TIMP3 were measured using enzyme-linked immunosorbent assay.Pearson/Spearman correlation was used to analyze the correlation of serum Ang and TIMP3 levels withβ-amyloid protein(Aβ)1-40,Aβ1-42,total Tau(t-Tau),phosphorylated Tau181(p-Tau181)levels and mini-mental state examination(MMSE)scores in AD patients.Receiver operating characteristic curve(ROC)curve was drawn to analyze the diagnostic value of serum Ang and TIMP3 levels for AD.Results The serum levels of Ang and TIMP3 in the AD group were significantly lower than those in the control group(P<0.05).Pearson/Spearman correlation analysis showed that serum Ang and TIMP3 levels in AD patients were negatively correlated with Aβ1-40 levels(r=-0.446,-0.465,P<0.05)and MMSE scores(r=-0.558,-0.607,P<0.05),and positively correlated with Aβ1-42 levels(r=0.443,0.437,P<0.05),but had no significant correlation with t-Tau and p-tau181 levels(P>0.05).ROC curve analysis showed that the AUCs of serum Ang and TIMP3 levels alone and in combination for the diagnosis of AD were 0.798,0.793 and 0.901,respectively,the sensitivity were 96.50%,67.13%and 74.13%,and the specificity were 56.36%,80.00%and 90.91%,respectively.The AUC of the combination of serum Ang and TIMP3 levels for the diagnosis of AD was greater than those of the two used alone(P<0.05).Conclusion The decrease of serum Ang and TIMP3 levels is related to the decline of cognitive function and Aβdeposition in AD patients,which can be used as auxiliary indicators for the diagnosis of AD,and the combined detection of

关 键 词：阿尔茨海默病 血管生成 血管抑制素 组织金属蛋白酶抑制剂3 Β-淀粉样蛋白 

分 类 号：R446.11[医药卫生—诊断学]