基于羧基改性荧光染料的手印显现方法  

Carboxyl-functionalized fluorescent dyes for latent fingerprint development

作　　者：张宏志 李明[1] 倪龙 袁传军 王猛[1,2] ZHANG Hong-zhi;LI Ming;NI Long;YUAN Chuan-jun;WANG Meng(College of Forensic Sciences,Criminal Investigation Police University of China,Shenyang 110035,China;Research Centre of Crime Governance in the New Era,Criminal Investigation Police University of China,Shenyang 110035,China)

机构地区：[1]中国刑事警察学院刑事科学技术学院,辽宁沈阳110035 [2]中国刑事警察学院新时代犯罪治理研究中心,辽宁沈阳110035

出　　处：《化学研究与应用》2023年第2期477-481,共5页Chemical Research and Application

基　　金：国家自然科学基金项目(21205139、21802169)资助;辽宁省教育厅科学研究经费项目(LJKZ0068、LJKZ0076)资助;辽宁省2020年度“百千万人才工程”项目资助。

摘　　要：本文提出了基于羧基改性荧光绿染料酰胺键合原理的手印显现方法。使用正硅酸四乙酯(TEOS)、3-氨基丙基三乙氧基硅烷(APTES)、丁二酸(SA)逐步对市售荧光绿染料的表面进行硅烷化、氨基化、羧基化修饰,并使用N-羟基琥珀酰亚胺(NHS)联合1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)对修饰的羧基进行活化,最终得到表面活化羧基改性的荧光绿染料。利用荧光绿材料表面活化的羧基与手印遗留物质中氨基酸的胺基发生酰胺键合反应,实现了潜在手印的高效显现。深入讨论了手印显现机理,详细考察了手印显现的对比度、灵敏度、选择性、适用性,客观对比了研究提出的新方法与传统手印显现方法之间的优劣。实验结果表明,对本研究中提出的基于酰胺键合原理的手印显现方法适用于常见非渗透性客体表面潜在手印的高质量显现,具有效果优良、省时高效、适用性广等优点。In this paper,an amidation-based approach for latent fingerprint development using green fluorescent dyes modified with activated carboxyl groups was proposed.Firstly,commercial green fluorescent dyes were carboxyl functionalized by treating with tetraethyl orthosilicate(TEOS),3-aminopropyltriethoxysilane(APTES),and succinic acid(SA),successively.Then,the carboxyl groups on surfaces of resultant products were activated by N-hydroxy succinimide(NHS)combined with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC).Finally,latent fingerprint development was successfully achieved via the rapid amidation between the activated carboxyl groups on surfaces of green fluorescent dyes and the amine groups from amino acids in fingerprint residues.The mechanism of this amidation-based fingerprint development was proposed.The contrast,sensitivity,selectivity,and applicability of this method were also investigated.In addition,the superiorities of this new method over the traditional methods were discussed in detail.Experimental results showed that our proposed amidation-based method could achieve a high performance in latent fingerprint development on common non-porous substrates,exhibiting high efficiency,good operability and wide applicability.

关 键 词：潜在手印 手印显现 荧光染料 表面修饰 羧基活化 

分 类 号：O657.3[理学—分析化学]