三肽D-Trp-Arg-Leu-NH2抑制B16黑色素瘤细胞黑色素合成的机制  

Inhibitory Effect of Tripeptide D-Trp-Arg-Leu-NH2 on Melanin Formation in B16 Murine Melanoma Cells

作　　者：王昭维 陈款民 支旭勃 张忠旗 梁强 赵金礼 WANG Zhaowei;CHEN Kuanmin;ZHI Xubo;ZHANG Zhongqi;LIANG Qiang;ZHAO Jinli(Shaanxi Huikang Bio-Tech Co.,Ltd.,Xi’an 710000,Shaanxi,China)

机构地区：[1]陕西慧康生物科技有限责任公司,陕西西安710000

出　　处：《香料香精化妆品》2023年第1期82-86,共5页Flavour Fragrance Cosmetics

基　　金：西安市博士后创新基地项目(编号市人社2019-683号)。

摘　　要：为了研究三肽D-Trp-Arg-Leu-NH2(dWRL)对小鼠B16细胞的酪氨酸酶(TYR)活性、黑色素合成及相关基因和蛋白表达的影响,探讨dWRL抑制黑色素生成的可能机制,我们体外培养小鼠B16细胞,采用MTT法测定细胞增殖情况、L-多巴氧化法测定TYR活性、氢氧化钠裂解法测定细胞黑色素含量、qRT-PCR和Western Blot法分别检测药物作用后B16细胞中小眼畸形相关转录因子(MITF)、TYR的mRNA和蛋白表达水平。结果显示三肽dWRL在试验浓度范围内可明显抑制α-黑色素细胞刺激素(α-MSH)诱导的B16细胞内TYR活性、黑色素合成量及MITF、TYR基因及蛋白的表达量,且呈浓度依赖性。所以三肽dWRL在体外能抑制B16细胞黑色素的合成,上述变化可能是通过下调MITF和TYR的基因转录和蛋白表达作用来完成。To study the effect of tripeptide D-Trp-Arg-Leu-NH2(dWRL) on cell viability, tyrosinase(TYR) activity and melanin production of B16 murine melanoma cells and clarify the mechanism of its stimulating effect on melanogenesis, four different concentrations of dWRL were incubated with B16 cells and then cell proliferation, melanin content, TYR activity were respectively measured. The protein and mRNA expression of melanogenesis key enzymes were investigated by the Western blot and RT-PCR. Results showed that in the range of experimental does, dWRL reduced the activity of the TYR and melanin content significantly in a dose dependent manner. The lower mRNA and protein expression of microphtalmiaassociated transcription factor(MITF) and TYR were also observed in a dose manner. It was concluded that the tripeptide dWRL can inhibite in B16 cells melanogenesis induced by α-melanocyte-stimulating hormone(α-MSH), which may be induced by reducing expression of MITF and TYR on mRNA and ptotein levels.

关 键 词：三肽D-Trp-Arg-Leu-NH2 B16细胞 α-黑色素细胞刺激素(α-MSH) 黑色素合成 酪氨酸酶 

分 类 号：TQ658[化学工程—精细化工]