5-氮杂胞苷对苦瓜果实成熟及转录表达谱的影响  

Effects of 5-azaC on fruit ripening and transcriptome patterns of bitter gourd(Momordica charantia L.)fruit

作　　者：郭金菊 吴廷全[1] 王茹芳 谭德龙[1] 曹海顺 王瑞[1] 张长远[1] GUO Jinju;WU Tingquan;WANG Rufang;TAN Delong;CAO Haishun;WANG Rui;ZHANG Changyuan(Institute of Facility Agriculture,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640,China)

机构地区：[1]广东省农业科学院设施农业研究所,广东广州510640

出　　处：《西北农林科技大学学报（自然科学版）》2023年第2期93-101,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：广东省现代农业产业技术体系创新团队项目(2021KJ110);广东省农业科学院院长基金项目(202028);广东省农业科学院创新基金项目(202149);广东省农业科学院学科团队建设项目(202130TD);广东省农业科学院科技创新战略专项资金(高水平农科院建设)-人才项目(R2018QD-037)。

摘　　要：【目的】研究5-氮杂胞苷(5-azaC)对苦瓜果实成熟和基因表达模式的影响,为解析苦瓜果实成熟的分子调控机制提供理论依据。【方法】以苦瓜(Momordica charantia L.)高世代自交系E12201-e1为材料,利用50 mmol/L 5-azaC处理绿熟期(授粉后18 d)苦瓜果实,以ddH2O处理为对照,观察处理组(TR)和对照组(CK)果实的表型变化,并利用RNA-seq技术比较TR和CK苦瓜果肉组织中的转录水平差异。【结果】50 mmol/L 5-azaC处理能明显延迟苦瓜果实成熟。RNA-seq获得的数据质量较高,可满足后续分析需求。通过筛选共获得1773个差异表达基因(DEGs),其中1007个上调基因,766个下调基因。GO和KEGG分析结果表明,DEGs主要富集在细胞组分和分子功能模块,217个DEGs共富集到93条代谢通路中,其中30个DEGs显著富集到光合作用和苯丙烷类生物合成通路。8个DEGs参与细胞壁代谢过程,其中6个基因在5-azaC处理中下调表达。共获得681个差异表达转录因子(TFs),分属于220个TF家族。12个DEGs的qRT-PCR结果与RNA-seq结果变化趋势一致。【结论】5-azaC处理抑制苦瓜果实成熟,并影响果实成熟相关基因的表达模式。【Objective】The effects of 5-azacytidine(5-azaC)on fruit ripening and gene expression patterns in bitter gourd were analyzed to provide basis for analyzing molecular regulatory mechanism of bitter gourd fruit ripening.【Method】The bitter gourd fruit at green mature stage(18 days after pollination)of high-generation inbred line E12201-e1 was used as material.After being treated with 50 mmol/L 5-azaC(TR)and ddH2O(CK),their phenotypes were observed and transcriptome patterns were compared using RNA-seq technique.【Result】The fruit ripening of bitter gourd was significantly delayed in 50 mmol/L 5-azaC treatment.RNA-seq obtained high quality data for subsequent analysis.A total of 1773 differentially expressed genes(DEGs)were identified,including 1007 up-regulated genes and 766 down-regulated genes.GO and KEGG enrichment analysis showed that DEGs were mainly concentrated in modules of cellular component and molecular function.A total of 217 DEGs were enriched in 93 metabolic pathways,and 30 DEGs from them were significantly enriched in photosynthesis and phenylpropanoid biosynthesis pathways.Eight DEGs were involved in cell wall metabolism,and 6 of them were down-regulated in 5-azaC treatment.A total of 681 differentially expressed transcription factors(TFs)belonging to 220 TF families were obtained.The qRT-PCR results of 12 DEGs were consistent with RNA-seq results.【Conclusion】The 5-azaC treatment could inhibit bitter gourd fruit ripening and affect gene expression patterns related to fruit ripening.

关 键 词：苦瓜 5-氮杂胞苷 转录组测序 果实成熟 

分 类 号：S642.501[农业科学—蔬菜学]