SlGH9-15 regulates tomato fruit cracking with hormonal and abiotic stress responsiveness cis-elements  

作　　者：LIN Hao-wei WU Zhen ZHOU Rong CHEN Bin ZHONG Zhao-jiang JIANG Fang-ling 

机构地区：[1]College of Horticulture,Nanjing Agricultural University/Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China,Ministry of Agriculture,Nanjing 210095,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第2期447-463,共17页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2019YFD100190200);the Jiangsu Agricultural Science and Technology Innovation Fund,China(CX(20)3101);a grant from the Fundamental Research Funds for the Central Universities,China(KYZZ2022004)。

摘　　要：Fruit cracking occurs easily during the late period of fruit development when plants encounter an unsuitable environment,dramatically affecting fruit production and marketing.This study conducted the bulked segregant RNA-Seq(BSR)to identify the key regulatory gene of fruit cracking in tomatoes.BSR-Seq analysis illustrated that two regions associated with irregularly cracking were located on chromosomes 9 and 11,containing 127 candidate genes.Further,through differentially expression analysis and qRT-PCR in cracking-susceptible and cracking-resistant genotypes,the candidate gene SlGH9-15(Solyc09g010210)with significantly differential expression levels was screened.Bioinformatics analysis of the GH9 gene family revealed that 20 SlGH9 genes were divided into three groups.The phylogenetic analysis showed that SlGH9-15 was closely related to cell wall construction-associated genes AtGH9B1,AtGH9B6,OsGH9B1,and OsGH9B3.The cis-acting elements analysis revealed that Sl GH9-15 was activated by various hormones(ethylene and ABA)and abiotic stresses.The expression pattern indicated that 13 SlGH9 genes,especially SlGH9-15,were highly expressed in the cracking-susceptible genotype.Its expression level gradually increased during fruit development and achieved maximum value at the red ripe stage.Additionally,the cracking-susceptible tomato showed higher cellulase activity and lower cellulose content than the cracking-resistant tomato,particularly at the red ripe stage.This study identified SlGH9-15 as a key gene associated with fruit cracking in tomatoes for the first time and gives new insights for understanding the molecular mechanism and complex regulatory network of fruit cracking.

关 键 词：tomato irregular cracking BSR-Seq GH9-15 

分 类 号：S641.2[农业科学—蔬菜学]