Characterization of subunits encoded by Sn RK1 and dissection of combinations among these subunits in sorghum(Sorghum bicolor L.)  

作　　者：XIAO Qian-lin HUANG Tian-hui ZHOU Chang CHEN Wei-xi CHA Jian-kui WEI Xi-mei XING Fang-yu QIAN Meng-ya MA Qian-nan DUAN Hong LIU Zhi-zhai 

机构地区：[1]College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第2期642-649,共8页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32001607);the Fundamental Research Funds for the Central Universities of Southwest University,China(SWU118087)。

摘　　要：Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic stresses.It plays a key role in distributing carbohydrates and sugar signal transporting.In the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of cereals.Expression results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in inflorescences.Results of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some organelles.Besides,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.

关 键 词：sorghum(Sorghum bicolor L.) SnRK1 expression analysis combination pattern 

分 类 号：S514[农业科学—作物学]